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目的探讨脾虚型功能性消化不良(FD)大鼠小肠组织葡萄糖转运体蛋白1(GLUT1)蛋白与mRNA表达及脾虚1号方的干预作用。方法 70只7日龄雄性SD大鼠随机分为正常对照组(n=10)、FD模型组(单模型组,n=10)、脾虚型FD模型组(n=50)。正常组给予2%蔗糖溶液灌胃,FD模型组和脾虚型FD模型组均给予0.1%蔗糖碘乙酰胺蔗糖溶液灌胃,0.2 m L/只·d,连续6d。脾虚型FD模型组正常饲料喂养至6周龄后叠加改良小平台站立,连续14 d;造模结束后随机分为双模型组、多潘立酮组和脾虚1号方低、中、高剂量组,每组10只,连同正常组、单模型组,每日分别给予蒸馏水10 m L/kg、多潘立酮3.125 mg/kg和脾虚1号方1.275、2.55、5.1 g/kg和灌胃14d。采用免疫组化、Western-blot和RT-PCR方法检测小肠组织GLUT1蛋白与mRNA表达。结果与正常组相比,双模型组大鼠小肠黏膜GLUT1蛋白平均光密度值、小肠组织GLUT1蛋白和mRNA表达量均降低(P<0.05,P<0.01);与双模型组相比,脾虚1号方低剂量组大鼠小肠黏膜GLUT1蛋白平均光密度值、小肠组织GLUT1蛋白和mRNA表达量均升高(P<0.01)。结论脾虚型FD大鼠存在GLUT1 mRNA及蛋白的表达量降低,脾虚1号方能够上调GLUT1 mRNA及蛋白的表达量。
Objective To investigate the protein and mRNA expression of GLUT1 in spleen deficiency type dyspepsia (FD) rats and the intervention of spleen deficiency No.1. Methods Seventy seven-day-old male SD rats were randomly divided into normal control group (n = 10), FD model group (n = 10) and FD model group (n = 50). The normal group was given 2% sucrose solution by gavage. FD model group and FD model group were given 0.1% sucrose solution of iodoacetamide sucrose solution, 0.2 m L / d · d for 6 days. Spleen-deficient FD model group was fed with normal diet until 6 weeks of age, and then the modified small platform was left standing for 14 days. After the model was established, the mice were randomly divided into two groups: model group, domperidone group and spleen deficiency 1, 10 rats in each group were given distilled water 10 m L / kg, domperidone 3.125 mg / kg and spleen deficiency No.1 1.275, 2.55, 5.1 g / kg and gavage respectively for 14 days, together with normal group and single model group. Immunohistochemistry, Western-blot and RT-PCR were used to detect the protein and mRNA expression of GLUT1 in small intestine. Results Compared with the normal group, the mean optical density of GLUT1 protein in small intestine mucosa and the expression of GLUT1 protein and mRNA in small intestine of model group decreased (P <0.05, P <0.01) The average optical density of GLUT1 protein in small intestine mucosa and the expression of GLUT1 protein and mRNA in small intestine of low dose group were significantly increased (P <0.01). Conclusions The expression of GLUT1 mRNA and protein in spleen deficiency type FD rats is decreased. Spleen deficiency type 1 can up-regulate the expression of GLUT1 mRNA and protein.