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Aim:To investigate the interaction between hydrogen sulfide(H_2S)/cystathion-ine γ-lyase(CSE)and carbon monoxide(CO)/heme oxygenase(HO)pathways inaortic smooth muscle cells(ASMC).Methods:The ASMCs were divided into thefollowing groups:(1)the control group;(2)the zinc protoporphyrin(ZnPP)20μmol/L group;(3)the propargylglycine(PPG)2 mmol/L,4 mmol/L and 10 mmol/Lgroups;and(4)the sodium hydrosulfide(NariS)1×10~(-5)mol/L,1×10~(-4)mol/L and1×10~(-3)mol/L groups.Each of the groups was further divided into 6h,12h,18hand 24h subgroups.The CO level,represented by carboxyhemoglobin(HbCO)content was measured using a spectrophotometric method and H_2S content wasdetected by a sensitive electrode method.CSE and HO-1 expressions were de-tected by Western blotting.Results:The H_2S content in the medium and CSEexpression by ASMC were markedly increased by ZnPP compared with the con-trol group.HbCO content in the medium and HO-1 expression by the ASMCstarted strengthening following 24h treatment with PPG at 2 mmol/L,but werefurther strengthened following 18h and 24h treatment with PPG at 4 mmol/Lcompared with the controls(P<0.01).PPG at 10 mmol/L increased the HbCO levelin the medium following 18h treatment and increased HO-1 expression by theASMC following 12h treatment.Moreover,NariS at 1×10~(-5)mol/L and 1×10~(-4)mol/Ldecreased the HbCO level in the medium and HO-1 expression by the ASMC after6h and 12h treatment,while NariS at 1×10~(-3)mol/L decreased them at all timepoints of the treatments.Conclusion:The results suggested that endogenousCO/HO and H_2S/CSE pathways inhibited each other in ASMC under physiologi-cal conditions.
Aim: To investigate the interaction between hydrogen sulfide (H_2S) / cystathion-ine γ-lyase (CSE) and carbon monoxide (CO) / heme oxygenase (HO) pathways in aortic smooth muscle cells (ASMC). Methods: The ASMCs were divided into (2) the zinc protoporphyrin (ZnPP) 20 μmol / L group; (3) the propargylglycine (PPG) 2 mmol / L, 4 mmol / L and 10 mmol / L groups; ) the sodium hydrosulfide (NariS) 1 × 10 ~ (-5) mol / L, 1 × 10 ~ (-4) mol / L and 1 × 10 ~ (-3) mol / L groups.Each of the groups was further divided The CO level, represented by carboxyhemoglobin (HbCO) content was measured using a spectrophotometric method and H_2S content wasdetected by a sensitive electrode method. CE and HO-1 expressions were de-tected by Western blotting. Results: The H_2S content in the medium and CSEexpression by ASMC were markedly increased by ZnPP compared with the con-trol group. HbCO content in the medium and HO-1 expression by the ASMCstarted strengthening following 24h treatment wi PPG at 10 mmol / L increased the HbCO level at the medium following 18h treatment and increased HO at PPG at 2 mmol / L, but the following 18h and 24h treatment with PPG at 4 mmol / Lcompared with the controls (P <0.01) -1 expression by the ASMC following 12h treatment. Moreover, NariS at 1 × 10 ~ (-5) mol / L and 1 × 10 ~ (-4) mol / Ldecreased the HbCO level in the medium and HO-1 expression by the ASMC after6h and 12h treatment, while NariS at 1 × 10 -3 mol / L decreased them at all timepoints of the treatments. Conclusion: The results suggested that endogenousCO / HO and H_2S / CSE pathways inhibited each other in ASMC under physiologi- cal conditions.