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目的：探讨三苯氧胺的不同剂量（0.1、1、10μmol／L）对人卵巢癌细胞增殖和凋亡的影响，为卵巢癌的内分泌治疗提供实验依据。方法：以体外培养人卵巢癌细胞系HO－8910为研究对象，用免疫组化SABC法检测增殖细胞核抗原（PCNA）在细胞中表达情况，DNA缺口原位末端标记方法（TUNEL）检测细胞凋亡。结果：三苯氧胺通过诱导细胞凋亡抑制卵巢癌细胞生长，至剂量依赖性。低浓度（≤1μmol／L）的三苯氧胺对PCNA表达的影响无统计学意义，高剂量（10μmol／L）可明显降低PCNA表达。结论：三苯氧胺抗肿瘤作用与剂量有关，低剂量通过诱导细胞凋亡发挥作用，而高剂量与抑制细胞增殖和诱导凋亡有关。
Objective: To investigate the effect of different doses of tamoxifen (0.1,1,10 μmol / L) on the proliferation and apoptosis of human ovarian cancer cells and provide experimental evidence for the endocrine therapy of ovarian cancer. Methods: Human ovarian cancer cell line HO-8910 was cultured in vitro. The expression of proliferating cell nuclear antigen (PCNA) in the cells was detected by immunohistochemical SABC method. Apoptosis was detected by DNA nick end labeling (TUNEL) . Results: Tamoxifen suppressed ovarian cancer cell growth by inducing apoptosis in a dose-dependent manner. The effect of low concentration (≤1μmol / L) of tamoxifen on PCNA expression was not statistically significant, high dose (10μmol / L) can significantly reduce the expression of PCNA. Conclusion: The antitumor effect of tamoxifen was dose-dependent. Low dose of tamoxifen could induce cell apoptosis, while high dose of tamoxifen could inhibit cell proliferation and induce apoptosis.