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目的 :观察马兜铃酸I(AristolochicAcidI)对肾小管上皮细胞超微结构的影响 ,并与庆大霉素所致损伤进行比较研究。 方法 :以体外培养的人近端肾小管上皮HKC细胞作为研究对象 ,分别给予马兜铃酸I(2 0 0、80 0、32 0 0 μg/L)和庆大霉素 (2 0 0、80 0、32 0 0mg/L) ,并设对照组 ,2 4h后检测细胞数量、细胞生存率、培养上清乳酸脱氢酶 (LDH)和N 乙酰 β D 氨基葡萄糖苷酶 (NAG)含量。细胞经处理后 ,采用透射电子显微镜观察细胞超微结构变化 ,单盲下随机计数 2 0个细胞 ,统计各组细胞超微结构差异。 结果 :2 4h后 ,在各组细胞数、细胞生存率、培养上清LDH和NAG酶等指标都尚无显著变化时 ,给药组细胞超微结构已经发生显著变化。马兜铃酸I组细胞以核结构变异为主 ,表现为核畸形、核仁缺失、巨核、小核、核膜增生卷曲等 ;高浓度组细胞同时具有少量线粒体肿胀等膜性结构变化。马兜铃酸I组中细胞核变异及膜性结构变异程度都与剂量成显著正相关。庆大霉素组细胞以溶酶体变化为主 ,产生髓样小体、残余小体等 ,并可见发育不良的线粒体等 ,变异程度与剂量成正相关。庆大霉素组核变异状况与正常对照组无显著差异 ,与药物剂量也无显著相关性。 结论 :马兜铃酸I对人肾小管上皮细胞超微结构的影响以核变异为主 ,在高
Objective : To observe the effect of aristolochic acid I (Aristolochic Acid I) on the ultrastructure of renal tubular epithelial cells, and to compare the damage caused by gentamycin. METHODS: Human proximal tubular epithelial HKC cells cultured in vitro were used as study subjects and were given aristolochic acid I (200, 800, 3200 μg/L) and gentamycin (200, respectively). 80, 32 0 0 mg/L), and set the control group, cell number, cell survival rate, culture supernatant lactate dehydrogenase (LDH) and N acetyl beta D glucosaminidase (NAG) content were measured after 24 hours. After the cells were treated, the ultrastructural changes of the cells were observed by transmission electron microscopy, and 20 cells were randomly counted under single blindness. The ultrastructure differences of the cells in each group were counted. RESULTS : After 24 hours, there were no significant changes in cell number, cell survival rate, culture supernatant LDH and NAG enzyme, etc., and the ultrastructure of cells in the treated group had changed significantly. The aristolochic acid group I cells were mainly composed of nuclear structure mutations, manifested as nuclear malformation, nucleoli loss, macronucleus, small nucleus, nuclear membrane hyperplasia, etc.; high concentration group cells also had a small amount of mitochondrial swelling and other membrane structure changes. There was a significant positive correlation between the degree of nucleus variation and membranous structure variation in the aristolochic acid I group and the dose. In the gentamicin group, lysosomes were the main changes, producing medulloid bodies, residual bodies, etc., and dysplastic mitochondria, etc., and the degree of variation was positively correlated with the dose. There was no significant difference between the gentamycin nuclear variation and the normal control group, and there was no significant correlation with the drug dose. Conclusion: The effect of aristolochic acid I on the ultrastructure of human renal tubular epithelial cells is mainly nuclear variation,