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不可逆电穿孔肿瘤消融的有效性与安全性已经过多年的临床应用而得到验证,新近发展起来的高频不可逆电穿孔肿瘤消融新方法兼具传统不可逆电穿孔的优势和缓解肌肉收缩的长处,然而目前对其作用机理还未深入研究。为此,从阻抗谱分析角度分析了高频不可逆电穿孔与传统不可逆电穿孔组织消融的差异性,研究了相同剂量下高频不可逆电穿孔与传统不可逆电穿孔消融新西兰大白兔肝脏组织引起的组织阻抗谱变化,并通过等效电路的拟合提取了阻抗谱变化中的信息。研究结果表明:脉冲处理后组织阻抗谱显著变化,阻抗模在中低频段显著减小,阻抗角在中高频段从负值向零值方向变化;通过等效电路拟合发现,处理后显著变化的是细胞外液等效电阻,由于细胞膜穿孔而增加了新的与细胞外液等效电阻并联的电流通路;脉冲剂量相同条件下,传统不可逆电穿孔脉冲作用引起的细胞外液等效电阻变化最大,高频不可逆电穿孔作用下,脉冲宽度越宽,细胞外液等效电阻变化就越显著。因此,组织阻抗谱变化及由它得到的等效电路元器件值变化,可以有效地反映电穿孔程度,有望成为临床应用中检测不可逆电穿孔治疗效果的有效方式。
The effectiveness and safety of irreversible electroporation tumor ablation have been validated over many years of clinical application. The newly developed high-frequency irreversible electroporation tumor ablation method combines the advantages of traditional irreversible electroporation with the advantages of mitigating muscle contraction, however, At present, its mechanism of action has not been studied in depth. Therefore, the difference between high frequency irreversible electroporation and traditional irreversible electroporation tissue ablation was analyzed from the point of view of impedance spectroscopy. The effects of high frequency irreversible electroporation and traditional irreversible electroporation on the liver tissue elicitation in New Zealand white rabbits Impedance spectrum changes, and through the equivalent circuit to extract the impedance spectrum changes in the information. The results showed that the impedance spectrum of the treated group changed significantly after pulse treatment, the impedance mode decreased significantly in the middle and low frequency range, and the impedance angle changed from negative value to zero value in the middle and high frequency range. The equivalent circuit fitting showed significant changes Of the extracellular fluid equivalent resistance, due to the cell membrane perforation increased with the extracellular fluid equivalent resistance in parallel with the current path; pulse dose under the same conditions, the traditional irreversible electroporation pulse caused by changes in the equivalent resistance of extracellular fluid Maximum, high frequency irreversible electroporation, the wider the pulse width, the more obvious changes in the equivalent resistance of the extracellular fluid. Therefore, the change of tissue impedance spectrum and the change of equivalent circuit components obtained from it can effectively reflect the degree of electroporation, which is expected to be an effective way to detect the effect of irreversible electroporation in clinical application.