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目的探讨维吾尔族和汉族子宫颈癌(CC)患者中肿瘤干细胞相关基因POU5F1和NANOG在mRNA水平表达的差异及其与临床病理的关系。方法采用实时荧光定量PCR法(RT-PCR),检测69例子宫颈癌组织和16例子宫颈癌组织及其相应的癌旁新鲜组织标本中POU5F1、NANOG在mRNA水平上的表达情况,了解其在维吾尔族和汉族间的表达差异。根据患者临床分期、肿瘤分化程度、淋巴结转移进行分组,比较不同亚组患者中维吾尔族和汉族患者间POU5F1、NANOG表达水平的差异性。结果子宫颈癌组织中POU5F1 mRNA、NANOG mRNA的表达水平均较癌旁组织高(P﹤0.05)。维吾尔族CC患者POU5F1 mRNA、NANOG mRNA表达水平均高于汉族CC患者(P﹤0.05)。临床分期为Ⅱa~Ⅲ期、淋巴结转移阴性时,维吾尔族与汉族CC患者子宫颈癌组织中POU5F1 mRNA表达水平比较,差异有统计学意义(P﹤0.05);临床分期为Ⅱa~Ⅲ期、中-高分化、淋巴结转移阴性时,维吾尔族与汉族CC患者子宫颈癌组织中NANOG mRNA表达水平比较,差异有统计学意义(P﹤0.05)。结论 CC中肿瘤干细胞相关基因POU5F1 mRNA和NANOG mRNA的表达在维吾尔族和汉族人群中存在差异性,而此种差异性在Ⅱa~Ⅲ期、中高分化、淋巴结转移阴性的CC中表现更加明显。
Objective To investigate the differences of mRNA expression of POU5F1 and NANOG in Uigur and Han patients with cervical cancer (CC) and their relationship with clinicopathological features. Methods The mRNA expression levels of POU5F1 and NANOG in 69 cases of cervical cancer and 16 cases of cervical cancer and their corresponding adjacent tissues were detected by real-time fluorescence quantitative PCR (RT-PCR), and their expression in Uighur Discrepancies between ethnic groups and Han nationality. The differences of POU5F1 and NANOG expression between Uygur and Han patients in different subgroups were compared according to the clinical stage, tumor differentiation and lymph node metastasis. Results The expression levels of POU5F1 mRNA and NANOG mRNA in cervical cancer tissues were higher than those in paracancerous tissues (P <0.05). The POU5F1 mRNA and NANOG mRNA expression levels in Uigur patients with CC were higher than those in Han patients with CC (P <0.05). The clinical stage of stage Ⅱa ~ Ⅲ, lymph node metastasis was negative, Uighur and Han CC patients with cervical cancer POU5F1 mRNA expression levels, the difference was statistically significant (P <0.05); clinical stage for stage Ⅱ a ~ Ⅲ, - There was significant difference in the expression levels of NANOG mRNA between Uighur and Han nationality patients with cervical cancer (P <0.05). Conclusion The expression of POU5F1 mRNA and NANOG mRNA in CC patients is different in Uygur and Han population, but this difference is more obvious in stage Ⅱa-Ⅲ, moderately well-differentiated and lymph node metastasis-negative CC.