论文部分内容阅读
目的探讨建立室性心动过速动物模型的有效方法。方法成年雄性SD大鼠30只,体重(300±35)g。均分为3组:(1)异丙肾上腺素组:10只,接受开胸法结扎左前降支造成急性心肌梗死模型,7d后行心室电刺激诱发VT,诱发时腹腔注射异丙肾上腺素,观察VT诱发情况,记录电生理数据并进行评分;(2)对照组:10只,接受开胸法结扎左前降支造成急性心肌梗死模型,7d后行心室电刺激诱发VT,诱发时腹腔注射NS,观察VT诱发情况,记录电生理数据并进行评分;(3)假手术组:10只,开胸后冠脉下穿线,但不结扎,饲养相同的时间后进行相同的研究。结果异丙肾上腺素组8/10只大鼠成功建立急性心肌梗死模型,其中1只因麻醉意外死亡,另外1只术中出现心室颤动(VF)死亡。其余经ECG、病理检查证实AMI模型成功。术后7d接受心脏电生理检查,运用程序或非程序电刺激方法,7只成功诱发出持续性VT,其中1只直接诱发出VF,1只诱发出VT不到30s便转变为心室扑动、VF后,2只均抢救无效死亡。对照组7/10只大鼠成功建立AMI模型,4只成功诱发出持续性VT,其中1只直接诱发出VF,抢救无效死亡。假手术组1只诱发出VT。结论运用开胸法结扎左前降支建立急性心肌梗死动物模型,腹腔注射异丙肾上腺素,通过程序电刺激方法可成功建立VT动物模型,成功率较高,容易推广。
Objective To explore an effective method to establish an animal model of ventricular tachycardia. Methods Thirty male SD rats were born weighing 300 ± 35 g. The rats were randomly divided into 3 groups: (1) Isoproterenol group: 10 rats underwent thoracotomy for left anterior descending coronary artery (LAD) model, and VT was induced by ventricular electrical stimulation after 7 days. Intraperitoneal injection of isoproterenol, (2) The control group: 10 rats underwent thoracotomy for ligation of the left anterior descending branch to establish the model of acute myocardial infarction; VT was induced on the 7th day after intraventricular electrical stimulation and the intraperitoneal injection of NS (3) Sham-operated group: 10 rats underwent percutaneous coronary intervention after thoracotomy, but without ligation, the same study was carried out after feeding the same time. Results 8/10 rats in isoproterenol group were successfully established acute myocardial infarction model. One died of anesthesia and the other died of ventricular fibrillation (VF). The remaining ECG, pathological examination confirmed the success of the AMI model. Seven days after operation, cardiac electrophysiological examination was performed. Seven patients successfully induced persistent VT using program or non-procedural electrical stimulation. One of them induced VF directly, one induced VT less than 30s and then changed to ventricular flutter. After VF, 2 were killed and died. In the control group, AMI model was successfully established in 7 of 10 rats. Four of them successfully induced persistent VT, one of them directly induced VF and the other died of rescue. One sham operation group induced VT. Conclusions Thoracotomy is performed by ligating the left anterior descending branch to establish an animal model of acute myocardial infarction. Intraperitoneal injection of isoproterenol is used to establish a VT animal model by electrical stimulation. The success rate is high and it is easy to popularize.