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提出了通过微生物封堵降低渗透率来提高波及效率的低成本方法。为了了解生物膜在孔隙介质中的生长和营养物消耗量、细菌生长数量和渗透率降低程度之间的关系,把培养物绿脓杆菌接种入贝雷砂岩岩心和玻璃珠人造岩心。通过培养基生长和渗透率变化趋势来跟踪需氧微生物生长。成像显示生长是颗粒包层,用细胞外物质充填孔喉和岩体,大大降低了玻璃珠的有效孔隙度。观测到渗透率平均降低了54%。通过示踪测试表征了生物膜生长前后玻璃珠人造岩心的流动特性。在含有粗玻璃珠层和细玻璃珠层的非均质玻璃珠人造岩心中,使流动有效地转向了玻璃珠人造岩心的较低渗透层。结果表明,微生物封塞是减小高渗透漏失层中流动从而提高波及效率的有效方法。
A low-cost method of improving sweep efficiency by reducing microbial permeability by plugging was proposed. In order to understand the relationship between biofilm growth and nutrient consumption in porous media, bacterial growth numbers and the degree of permeability reduction, cultures of Pseudomonas aeruginosa were inoculated into Berea sandstone cores and glass bead artificial cores. Aerobic microbial growth is tracked by trends in media growth and permeability. Imaging shows that the growth is particle-coated, filling pore throats and rock mass with extracellular material, greatly reducing the effective porosity of the glass beads. Permeability was observed on average by 54%. The flow characteristics of glass bead artificial cores before and after biofilm growth were characterized by tracing tests. In heterogeneous glass bead artificial cores containing coarse glass bead layers and fine glass bead layers, the flow is effectively diverted to the lower permeable layers of the glass bead artificial cores. The results show that microbial plugging is an effective way to reduce the flow in the high-permeability layer and improve the sweep efficiency.