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目的考察参芪扶正注射液(SQFZ)对环磷酰胺(Cy)处理后小鼠肠道相关淋巴组织(GALT)B细胞数量恢复的影响。方法将BALB/c小鼠随机分为正常对照组、Cy组和SQFZ组。Cy组和SQFZ组小鼠均腹腔注射Cy(100 mg/kg),对照组给予等量生理盐水;24 h后,SQFZ组小鼠灌服SQFZ 1 m L/只,其余2组给予等量的生理盐水,每天灌胃1次连续10 d;每天记录小鼠体质量。给药第10天处死小鼠,测定胸腺指数和脾指数;流式细胞术检测肠系膜淋巴结(MLN)和Peyer集合淋巴结(PP)淋巴细胞中B细胞比例及股骨骨髓细胞周期。体外实验中,小鼠淋巴细胞经SQFZ处理后,MTT法检测细胞活性,流式细胞术检测B细胞表达CD86分子水平;羧基荧光素二乙酸盐琥珀酰亚胺酯(CFSE)标记法检测B细胞增殖。结果 SQFZ减少Cy处理小鼠体质量的下降,促进胸腺指数、脾指数、MLN和PP中B细胞数量恢复,但未见明显改善骨髓抑制状态。体外实验结果表明SQFZ可以增加小鼠淋巴细胞活性,提高小鼠B细胞活化和增殖比例。结论 SQFZ促进Cy处理后小鼠GALT中B细胞数量恢复,可能通过促进B细胞增殖而发挥作用。
Objective To investigate the effect of Shenqi Fuzheng Injection (SQFZ) on the recovery of intestinal lymphoid tissue (GALT) B cells in mice treated with cyclophosphamide (Cy). Methods BALB / c mice were randomly divided into normal control group, Cy group and SQFZ group. The mice in Cy group and SQFZ group were given intraperitoneal injection of Cy (100 mg / kg), while the control group were given the same amount of normal saline. After 24 hours, mice in SQFZ group were given SQFZ 1 m L / Saline, gavage once a day for 10 consecutive days; record the body weight of mice every day. The mice were sacrificed on the 10th day after administration, and the thymus index and spleen index were measured. The proportion of B cells in the mesenteric lymph nodes (MLNs) and Peyer’s lymphocytes (PPs) and the cell cycle of femur bone marrow were detected by flow cytometry. In vitro, the lymphocytes of mice were treated with SQFZ, the cell viability was detected by MTT assay, the level of CD86 was detected by flow cytometry, the level of CD86 was detected by CFSE labeling Cell Proliferation. Results SQFZ decreased the body weight of Cy-treated mice and promoted the recovery of B cells in thymus index, spleen index, MLN and PP, but no significant improvement of myelosuppression. In vitro experimental results show that SQFZ can increase lymphocyte activity in mice and increase the proportion of mouse B cell activation and proliferation. Conclusion SQFZ can promote the recovery of B cells in GALT mice after Cy treatment, which may play a role in promoting B cell proliferation.