论文部分内容阅读
目的:研究洪连对α-异硫氰酸萘酯(ANIT)所致黄疸型肝损伤小鼠的保护作用,并探讨其作用机制。方法:采用灌胃ANIT(100 mg·kg-1)花生油溶液造成小鼠急性肝损伤模型,连续给药7 d,末次给药1 h后取血、肝脏,检测血清中谷氨酸-丙酮酸转氨酶(ALT),天门冬氨酸转氨酶(AST),总胆汁酸(TBA),总胆红素(TBIL)和肿瘤坏死因子-α(TNF-α)的含量;检测肝组织匀浆中超氧化物歧化酶(SOD),丙二醛(MDA),谷胱甘肽过氧化物酶(GSH-Px)的含量,PCR检测肝组织中TNF-αmRNA的表达量,苏木素-伊红(HE)染色观察肝组织病理变化。结果:与模型组比较,洪连(1.0,2.0,4.0 g·kg-1)可显著降低肝损伤小鼠血清ALT,AST,TBIL和TNF-α的水平和肝匀浆中MDA含量,同时显著升高小鼠肝匀浆中GSH-Px的含量,洪连(2.0,4.0 g·kg-1)可显著降低肝损伤小鼠血清TBA的水平和显著升高肝匀浆中SOD含量(P<0.01),洪连(1.0 g·kg-1)组TBA,SOD含量与模型组比较无统计学差异。洪连提取物各剂量组均可显著降低肝组织中TNF-αmRNA相对表达量(P<0.01)。病理切片显示洪连(2.0,4.0 g·kg-1)组能显著改善肝组织的病理变化。结论:洪连对ANIT所致小鼠黄疸型肝损伤有保护作用。
Objective: To investigate the protective effect of Honglian on mice with icteric liver injury induced by α-isothiocyanate (ANIT) and its mechanism. Methods: Acute liver injury model was induced in mice by intragastric administration of ANIT (100 mg · kg-1) peanut oil solution for 7 days. Blood and liver were harvested at 1 h after the last administration. Glutamate-pyruvate aminotransferase (ALT), aspartate aminotransferase (AST), total bile acid (TBA), total bilirubin (TBIL) and tumor necrosis factor-α (TNF-α) were measured. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were detected by enzyme-linked immunosorbent assay. The expression of TNF-α mRNA in liver tissue was detected by PCR. Histopathological changes. Results: Compared with the model group, Honglian (1.0,2.0,4.0 g · kg -1) significantly reduced the level of serum ALT, AST, TBIL and TNF-α and the content of MDA in liver homogenate Honglian (2.0,4.0 g · kg-1) could significantly reduce the level of serum TBA and increase the content of SOD in liver homogenate (P < 0.01). The contents of TBA and SOD in Honglian (1.0 g · kg -1) group were not significantly different from the model group. Each dose of Hong Lian extract could significantly reduce the relative expression of TNF-α mRNA in liver tissue (P <0.01). Histopathological examination showed that Hong Lian (2.0, 4.0 g · kg -1) group could significantly improve the pathological changes of liver tissue. CONCLUSION: Honglian has a protective effect on jaundiced liver injury induced by ANIT in mice.