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目的 :通过对导光纤维引起的兔眼视网膜光损伤 ,制造一种光损伤模型 ,为视网膜光损伤防治的研究提供一种有效的手段。方法 :把 40 5nm波长的单色滤光片 ,置于玻璃体切割机的光源处 ,使单色光从导光纤维中导出 ,并用照度计测出光强度。将导光纤维插入兔眼内 ,根据光斑直径大小来决定导光纤维距照射部位的距离 ,以保证照射距离相等 ,所有兔的巩膜切口和照射部位均保持一致。选择一定的光能量照射兔眼 ,制成兔眼的视网膜光损伤模型。光照后 3天 ,进行眼底检查并拍片。然后将兔处死 ,剪下照射部位 ,做光、电镜检查。结果 :眼底镜检查可见光照射部位的视网膜水肿和脱色索。光、电镜检查可见光感受器细胞损伤的改变。在光镜下 ,随着能量的增加损伤程度也随之增加 ;在电镜下 ,因能量不同而引起的损伤程度的差别不明显。结论 :导光纤维可以引起兔眼的视网膜光损伤。采用导光纤维制成的光损伤模型可进行有害波长的筛选。
OBJECTIVE: To create a photodamage model of rabbit retinal photodamage caused by optical fiber and to provide an effective method for the prevention and treatment of retinal photodamage. Methods: The monochromatic filter of 40 5nm wavelength was placed on the light source of the vitrectomy machine, monochromatic light was led out of the light guide fiber, and the light intensity was measured by illuminometer. The guide fiber inserted into the rabbit eye, according to the size of the spot diameter to determine the distance from the radiation guide fiber location to ensure that the irradiation distance is equal, all rabbits scleral incision and irradiation sites are consistent. Select a certain amount of light energy irradiation rabbit eyes, rabbits made of retinal light damage model. 3 days after light, fundus examination and filming. Then the rabbits were sacrificed, cut off the irradiation site, light, electron microscopy. Results: The retinal edema and decolorization of the visible light irradiation site were examined by ophthalmoscopy. Light and electron microscopy were used to examine the changes of photoreceptor cell damage. Under the light microscope, as the energy increases, the degree of damage also increases; under the electron microscope, the difference in the degree of damage caused by different energy is not obvious. Conclusion: Light guide fibers can cause retinal light damage in rabbit eyes. Optical damage models made of light-guiding fibers allow screening of harmful wavelengths.