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对杉木种子园中51个无性系及其不同分株的过氧化物酶,进行了聚丙烯酸胺凝胶电泳分析.酶谱图式证明,同一无性系的不同分株,酶谱表型具有相对的稳定性和一致性.明显的酶谱差异,为鉴定杉木无性系提供了可靠的生化指标.采用酶谱距离,将系间差异数量化,借以比较其分岐程度.文章最后讨论了酶谱资料在研究杉木种子园及其无性系中的作用.
Peroxidase of 51 clones and their different ramets in Chinese fir seed orchard was analyzed by polyacrylamide gel electrophoresis.The zymogram of zymogram indicated that the different ramets and zymogram phenotypes of the same clones had relative The stability and consistency.The obvious difference of zymogram provided a reliable biochemical index for the identification of Chinese fir clones.Using the distance of zymogram to quantify the differences among the lines to compare their degree of differentiation.This article discussed the zymogram In the study of Chinese fir seed orchard and its role in cloning.