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目的:观察扶肾降浊方对肾间质纤维化大鼠的生化指标,肾组织形态及纤维化因子转化生长因子-β1(TGF-β1),肝细胞生长因子(HGF),Ⅰ型胶原(ColⅠ)蛋白表达的影响。方法:在系膜增生性肾炎(Ms PGN)的基础上,延长造模至12周,使其自然发展成肾间质纤维化模型,24只大鼠随机分为正常组、模型组、扶肾降浊方中药组、贝那普利西药组,分别检测大鼠血清尿素氮(BUN),血清肌酐(SCr),测定24 h蛋白尿,在光镜下观察肾组织切片病理改变,运用免疫组化法观察肾组织切片中TGF-β1,HGF,ColⅠ蛋白的表达情况。结果:通过对大鼠血清BUN,SCr,24 h蛋白尿定量的检测和肾组织形态的观察,模型组较正常组有明显的肾间质损伤(P<0.01),中药组和贝纳普利组损伤程度均减轻(P<0.01);第12周末,造模各组肾小管间质TGF-β1和ColⅠ蛋白表达均高于正常组(P<0.01),扶肾降浊方组及贝纳普利组均显著低于模型组(P<0.01);而模型组HGF蛋白表达低于正常组(P<0.01),扶肾降浊方组及贝纳普利组均高于模型组(P<0.01)。结论:扶肾降浊方可抑制TGF-β1和ColⅠ蛋白表达,促进HGF蛋白的升高,对肾间质纤维化有明显的保护作用。
OBJECTIVE: To observe the effects of Fu Shen Jiang Zhuo Fang on renal biopsy, renal morphology and the expressions of transforming growth factor-β1 (TGF-β1), hepatocyte growth factor (HGF), collagen type Ⅰ Col I) protein expression. Methods: On the basis of mesangial proliferative glomerulonephritis (Ms PGN), the model was extended to 12 weeks and then developed into a model of renal interstitial fibrosis. 24 rats were randomly divided into normal group, model group, The rats in Jiangzhuo decoction group and benazepril western medicine group were used to detect the serum urea nitrogen (BUN) and serum creatinine (SCr) respectively, and the proteinuria of 24 h was measured. The pathological changes of renal tissue sections were observed under light microscope. The expression of TGF-β1, HGF and ColⅠ in renal tissue sections was observed by immunohistochemistry. Results: Compared with normal group, the model group showed obvious renal interstitial injury (P <0.01) by quantitative detection of proteinuria of BUN, SCr, 24 h in rat serum and morphological observation of kidney. (P <0.01). At the end of the 12th week, the expression of TGF-β1 and ColⅠ in the tubulointerstitial of each model group was higher than that in the normal group (P <0.01) (P <0.01), while the expression of HGF protein in the model group was lower than that in the normal group (P <0.01), and that in the model group (P <0.01) <0.01). Conclusion: Fufang Jiangzhuo Decoction can inhibit the expression of TGF-β1 and ColⅠprotein, promote the increase of HGF protein, and have a significant protective effect on renal interstitial fibrosis.