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本实验采作细胞培养技术，从喉癌患者颈廓清淋巴结中提取的巨噬细胞（Mphi）作效应细胞，喉癌患者自体癌细胞及K562细胞作靶细胞，分别混合培养（两组均加PHA作为刺激原），结果表明：喉癌患者颈部肿大淋巴结Mphi对自体癌细胞（35例）及K562细胞（30例）均有杀伤能力；此外，我们还对比了未转移淋巴结（N0组，18例）与转移淋巴结（N+组，17例）MPhi杀伤能力，结果N0组高于N+组，差异非常显著（P＜0．01）。结果提示，喉癌患者颈部引流淋巴结中尚存在着免疫活性细胞，并具有肮肿瘤免疫潜能，该实验为临床合理实施颈廓清术及将活化的Mphi应用于肿瘤免疫治疗提供理论依据。
In this experiment, cell culture technique was used to collect Mphi cells from efferent cells, autologous cancer cells and K562 cells as target cells extracted from cervical lymph nodes in patients with laryngeal carcinoma, and mixed culture (PHA As a stimulant. The results showed that Mphi of cervical lymph nodes in patients with laryngeal carcinoma had cytotoxicity to autologous cancer cells (35 cases) and K562 cells (30 cases). In addition, we also compared non-metastatic lymph nodes (N0 group, 18 cases) and MPIL (17 cases) in lymph node metastasis (N + group). The result showed that the MPHI in N0 group was higher than that in N + group, the difference was significant (P <0.01). The results suggest that there are still immunocompetent cells in the cervical drainage lymph nodes of patients with laryngeal cancer and have the potential of tumor immunostaining. This experiment provides a theoretical basis for the clinical practice of neck dissection and the application of activated Mphi in tumor immunotherapy.