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[目的]观察新生鼠窒息后不同时间段脑组织NF-κB动态变化,以探讨NF-κB信号途径在新生鼠窒息后缺氧缺血性脑损伤中的作用。[方法]出生7d新生鼠随机分为对照组(A组)和窒息组(B组);制备新生鼠窒息模型,并于窒息后1、3、5和7d取脑组织行石蜡切片。采用免疫组织化学方法检测窒息新生鼠脑组织NF-κB p65活性表达水平。[结果]窒息组各组脑组织NF-κB的表达均较A组显著增强(P﹤0.05),以5d时升高最明显,并伴有神经细胞坏死及凋亡现象。[结论]NF-κB可能通过炎症反应机制介导窒息后脑损伤过程。
[Objective] To observe the dynamic changes of NF-κB in brain tissue of neonatal rats after asphyxia in order to investigate the role of NF-κB signaling in hypoxic-ischemic brain damage after neonatal asphyxia. [Method] Newborn rats were randomly divided into control group (group A) and asphyxia group (group B) on the 7th day after birth. The asphyxia model of neonatal rats was prepared. The brain tissues were paraffin sectioned at 1, 3, 5 and 7 days after asphyxia. Immunohistochemistry was used to detect the expression of NF-κB p65 in neonatal rats with asphyxia. [Results] The expression of NF-κB in brain tissue of asphyxia group was significantly higher than that of group A (P <0.05), and increased most obviously on the 5th day, accompanied by the necrosis and apoptosis of nerve cells. [Conclusion] NF-κB may mediate post-asphyxial brain injury process through inflammatory response mechanism.