【摘 要】
:
目的 构建变应性鼻炎小鼠动物模型,经鼻腔滴入及腹腔注射CCR3单克隆抗体,观察其对嗜酸性粒细胞、肥大细胞及相关炎症介质的作用,研究CCR3单克隆抗体在过敏性鼻炎中的作用及机制.方法 将6~8周雄性BALB/c分为四组:①正常对照组(normal组),②过敏性鼻炎组(AR组),③CCR3mAb腹腔注射组(CCR3mAb IP组),④CCR3mAb鼻腔滴入组(CCR3mAb ND组),每组小鼠5只,共20只.以卵清白蛋白(OVA)致敏,构建过敏性鼻炎小鼠模型,采用鼻腔滴入及腹腔注射两种途径,将CCR3单克隆抗
【机 构】
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南昌大学第二附属医院耳鼻咽喉头颈外科,江西,330000
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目的 构建变应性鼻炎小鼠动物模型,经鼻腔滴入及腹腔注射CCR3单克隆抗体,观察其对嗜酸性粒细胞、肥大细胞及相关炎症介质的作用,研究CCR3单克隆抗体在过敏性鼻炎中的作用及机制.方法 将6~8周雄性BALB/c分为四组:①正常对照组(normal组),②过敏性鼻炎组(AR组),③CCR3mAb腹腔注射组(CCR3mAb IP组),④CCR3mAb鼻腔滴入组(CCR3mAb ND组),每组小鼠5只,共20只.以卵清白蛋白(OVA)致敏,构建过敏性鼻炎小鼠模型,采用鼻腔滴入及腹腔注射两种途径,将CCR3单克隆抗体应用于小鼠过敏性鼻炎模型中.计数各组小鼠末次鼻腔激发后10分钟内抓鼻数和喷嚏数,收集各组小鼠的鼻黏膜组织标本,应用HE染色检测鼻黏膜组织形态变化;应用免疫组化检测鼻黏膜嗜酸性粒细胞及肥大细胞的浸润情况;应用Elisa方法测定小鼠鼻黏膜中IL-2、TNF-α、IL-10及组胺和IgE的浓度及变化.结果 ①HE染色:经CCR3mAb腹腔注射组及CCR3mAb鼻腔滴入组,小鼠鼻黏膜组织的炎症浸润及黏膜结构紊乱情况均较过敏性鼻炎组明显好转.②免疫组化:CCR3mAb腹腔注射组及CCR3mAb鼻腔滴入组,小鼠鼻黏膜组织中炎性细胞较过敏性鼻炎组减少.③Elisa分析发现在CCR3mAb腹腔注射组及CCR3mAb鼻腔滴入组,小鼠鼻黏膜组织中的IL-2、TNF-α、IL-10组胺及IgE水平均较过敏性鼻炎组明显降低,差异具有统计学意义(P<0.05).结论 CCR3 mAb对过敏性鼻炎小鼠模型的炎症抑制有效,可通过鼻腔黏膜及腹腔吸收两种途径产生作用,并通过抑制鼻腔黏膜的相关炎性介质而减轻鼻腔黏膜的炎症,从而减轻过敏性鼻炎的症状.
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