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根据ＴＣＲδ基因的Ｖδ２和Ｄδ３、Ｊδ之间内含子序列设计两端引物，建立了ＴＣＲδ基因的Ｖδ２～Ｄδ３不完全重排半嵌套式ＰＣＲ检测方法，对５７例非霍奇金淋巴瘤（ＮＨＬ）进行分析，５２．６％获得阳性结果；而正常人外周血淋巴细胞、淋巴结反应性增生及淋巴结转移性癌均为阴性，在此基础上，又建立了ＰＣＲ－ＳＳＣＰ银染检测方法进行分析，阳性病例呈现一系列清晰条带，而阴性者呈现模糊涂片现象，ＰＣＲ－ＳＳＣＰ两种方法分析的结果一致，说明二者均可应用于ＴＣＲδ基因重排研究，区别淋巴系细胞的单克隆性或多克隆增生，确定肿瘤的克隆性，可以作为ＮＨＬ的一种基因标志用于淋巴瘤的诊断，并有助于分析淋巴细胞的分化情况。
Based on the intron sequences between Vδ2 and Dδ3 and Jδ of TCRδ gene, primers at both ends were designed. A semi-nested PCR method for incomplete rearrangement of Vδ2 to Dδ3 of TCRδ gene was established, and 57 cases of non-Hodgkin’s lymphoma were identified. NHL) analysis showed that 52.6% obtained positive results. In normal subjects, peripheral blood lymphocytes, reactive hyperplasia of lymph nodes, and metastatic lymph node metastases were negative. On this basis, a PCR-SSCP silver staining assay was established. Analysis, positive cases presented a series of clear bands, while negative ones presented fuzzy smears. The results of the two methods of PCR-SSCP were consistent, indicating that both of them can be applied to the study of TCR δ gene rearrangement, distinguishing lymphocyte cells Clone or polyclonal proliferation, to determine the clonality of the tumor, can be used as a gene marker of the NHL for the diagnosis of lymphoma, and help analyze the lymphocyte differentiation.