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AIM.To study the distribution of the constitutive nitric oxidesynthase(NOS)in the jejunum of adult rat.METHODS:The distribution of endothelial NOS(eNOS)wasdetected by immunohistochemistry.Immunofluorescencehistochemical dual staining technique ware used forstudying the distribution of neuronal NOS(nNOS)andeNOS.The dual stained slides were observed under aconfocal laser scanning microscope.RESULTS:Positive neuronal NOS(nNOS)and endothelialNOS(eNOS)cells were found to be distributed in laminapropria of villi,and the epithelial cell was not stained,eNOSwas mainly located in submucosal vascular endothelia,while nNOS was mainly situated in myenteric plexus.Somecells in the villi had both nNOS and eNOS.More than 80%of the cells ware positive for both nNOS and eNOS,the restcells ware positive either for nNOS or for eNOS.CONCLUSION:The two constitutive nitric oxide synthesesare distributed differently in the jejunum of rat.nNOSdistributed in myenteric plexus is a neurotransmitter in thenon-adrenergic non-cholinergic(NANC)inhibitory nerves.eNOS distributed in endothelial and smooth muscle cells ofblood vessels plays vasodilator role.eNOS and nNOS arecoexpressed in some cells of lamina propria of villi.NOgenerated by those NOS is very important in thephysiological and pathological process of small intestine.
AIM. Study of the distribution of the constitutive nitric oxides synthase (NOS) in the jejunum of adult rat. METHODS: The distribution of endothelial NOS (eNOS) wasdetected by immunohistochemistry. Immunofluorescencehistochemical dual staining technique ware used forstudying the distribution of neuronal NOS (nNOS) RESULTS: Positive neuronal NOS (nNOS) and endothelialNOS (eNOS) cells were found to be distributed in laminapropria of villi, and the epithelial cell was not stained, eNOSwas mainly located in submucosal vascular endothelia, while nNOS was mainly situated in myenteric plexus. Cells in the villi had both nNOS and eNOS. More than 80% of the cells ware positive for both nNOS and eNOS, the rest cells were either positive for nNOS or for eNOS. CONCLUSION : The two constitutive nitric oxide synthesesare distributed differently in the jejunum of rat. NNOSdistributed in myenteric plexus is a neurotransmitter in thenon-adr energic non-cholinergic (NANC) inhibitory nerves. ENOS distributed in endothelial and smooth muscle cells of blood vessels plays vasodilator role. NOS and nNOS arecoexpressed in some cells of lamina propria of villi. NOgenerated by those NOS is very important in the physiology and pathological process of small intestine