本研究运用基因芯片技术,对双胚苗水稻SARⅡ-628品系中的单倍体及其对应二倍体的RNA水平的表达变化情况进行对比分析,发现单倍化后基因表达迅速发生了不同程度的变异,主要结果如下:(i)单倍化后,表达变化的序列占水稻总探针序列的2.47%,随机分布在水稻的12条染色体上,激活的序列数量多于沉默的;(ii)有33个序列在染色体上的分布具有成簇分布的特点;(iii)对单倍化中已经预测功能的575条序列进行功能分类,发现都涉及了生物过程、细胞成份和分子功能这3个方面.SNF2家族中控制转录调节的位点表达下降居多;与DNA重组修复相关的RAD54家族中只有2个位点分别发生了表达上升和降低的变化;(iv)采用RT-PCR验证基因芯片的结果,总体上83.78%序列表达结果与芯片结果相符;任选7个极端(激活和沉默)表达方式的序列,其表达结果与芯片相符率达到91.86%. In this study, gene chip technology was used to compare the changes of the RNA level between haploid and its diploid in the strain of SARII-628 double blastoderm rice. It was found that the gene expression was rapidly changed to some extent The main results are as follows: (i) After the haplotype, the sequence of the expression change accounted for 2.47% of the total probe sequence of rice, randomly distributed on 12 chromosomes of rice and the number of activated sequences was more than that of silence; (ii) ) There are 33 sequences that are clustered across the chromosomes; (iii) Functional classification of the 575 sequences that have been predicted for haplotype has been found to involve biological processes, cellular components, and molecular functions On the other hand, only two of the RAD54s, which are related to DNA recombination and repair, showed up-regulated and down-regulated, respectively. (Iv) RT-PCR was used to verify that the gene chip The results showed that 83.78% of the sequences were consistent with the results of the chips. The sequences of the seven extreme (activation and silencing) expressions were selected and their expression rates were 91.86% identical to the chips.