用高效液相色谱(HPLC)法测定了31个南瓜品种果肉中β－胡萝卜素的含量.取干粉样品用石油醚提取,旋转蒸干,正已烷定容,然后进行HPLC测定.分析柱为NOVA－PAKC18柱,流动相为90％甲醇与乙酸乙酯洗脱梯度,在450NM处检测.测得品种间β－胡萝卜素的含量有显著差异,西班牙南瓜中β－胡萝卜素的含量8.735MG/100G(鲜重),远远高于其它南瓜品种,甚至高于胡萝卜中β－胡萝卜素的含量,为富含β－胡萝卜素的高保健南瓜.“,”β-carotene from pulps of 31 varieties of pumpkin were determined by reversed phase high performance liquid chromatography (RP HPLC) with ultraviolet (UV) detector(254nm),a C_18 column and an eluant of 90%methanol and ethyl acetate.Dried powder samples were extracted by petroleum ether,evaporated to dryness and then dissolved by hexane to a final ratio of 1g of dried sample per 10ml hexane.The hexane solutions were analysed by HPLC.Difference of contents of β carotene between pumpkin varieties were found ...