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热胁迫的不利影响是实现小麦遗传生产潜力的最大限制因素。本项研究以麦苗为材料探讨了植物“热冲击蛋白质”(HSP)合成中的温度诱导反应特性。各HSP的测定结果发现,在非致死温度下开花小麦植株的旗叶与生产中所遇到的情况相似。HSP纵剖面图用~(35)S-蛋氨酸标记产物的等电聚焦法和聚丙烯酰胺凝胶电泳法来显示HSP纵剖面图特点,该标记产物由聚(A)~+RNA体外转移。聚(A)~+RNA是从分别暴露于22、25、28、31、34和37℃2h的幼苗叶片中以及从置于32℃下2h的旗叶中获得的。各种HSP的合成是在28—31℃间小麦处于热冲击状态下的幼苗叶片组织中和在32℃下的旗叶中发现的。通过运用HSP16.9和HSP70~(32)P标记探针的northern印迹分析进一步证实上述观察结果,表明当叶片温度比其适宜生长温度(18—23℃)高出10℃时,小麦对热胁迫的反应表现为合成高分子量和低分子量的HSP。发现在幼苗和开花植株的旗叶上合成高分子量和低分子量的HSP,这意味着HSP是在叶温达到对其生长和发育有害的水平之前合成的。
The adverse effects of heat stress are the most limiting factor in realizing the potential of wheat genetic production. In this study, we used wheat germplasm as material to investigate the temperature-induced reaction in the synthesis of plant “heat shock protein” (HSP). The determination of each HSP found that the flag leaf of a flowering wheat plant at a non-lethal temperature is similar to that encountered in production. HSP longitudinal profile shows the longitudinal profile of HSP using isoelectric focusing of ~ (35) S-methionine-labeled product and polyacrylamide gel electrophoresis, which was transferred in vitro from poly (A) ~ + RNA. Poly (A) ~ + RNA was obtained from seedling leaves exposed at 22, 25, 28, 31, 34 and 37 ° C for 2 h, respectively, and from flag leaves placed at 32 ° C for 2 h. The synthesis of various HSPs was found in the seedling leaf tissue of wheat under thermal shock at 28-31 ° C and in the flag leaf at 32 ° C. The above observations were further confirmed by northern blot analysis using HSP16.9 and HSP70- (32) P-labeled probes, indicating that when the leaf temperature is 10 ° C above its optimum growth temperature (18-23 ° C) The reaction shows the synthesis of high molecular weight and low molecular weight HSP. It was found that high molecular weight and low molecular weight HSPs were synthesized on the flag leaves of seedlings and flowering plants, meaning that HSPs were synthesized before leaf temperature reached levels that were detrimental to its growth and development.