[目的]探讨ZNF488基因对鼻咽癌细胞HONE1生物学行为的影响。[方法]构建携带空载体、人ZNF488基因的逆转录病毒质粒pMSCV和pMSCV-ZNF488,以磷酸钙法转染293FT细胞并收集病毒液,感染鼻咽癌细胞株HONE1,经嘌呤霉素筛选及Western Blot鉴定,建立稳定表达ZNF488的HONE1-pMSCV-ZNF488和空载体HONE1-pMSCV的细胞株。应用噻唑盐(MTT)法、平板克隆形成实验检测ZNF488过表达对HONE1细胞生长增殖的影响,同时应用流式细胞术检测ZNF488对细胞周期增殖指数的影响。划痕实验及Transwell体外侵袭实验观察ZNF488对HONE1迁移侵袭能力的影响。Western Blot检测上皮—间质转化(EMT)相关蛋白及抑癌基因PTEN的表达情况。[结果 ]成功建立稳定表达ZNF488的HONE1-pMSCV-ZNF488细胞株,MTT法及平板克隆形成实验结果显示,与空载体组HONE1-pMSCV细胞相比,HONE1-pMSCV-ZNF488细胞增殖及克隆形成能力均增强。流式细胞术显示过表达ZNF488细胞S期百分比较空载体组高,增殖指数增加。划痕实验、Transwell体外侵袭实验示ZNF488能够显著增加细胞的迁移、侵袭能力。Western Blot检测发现上皮标志E-Cadherin、α-Catenin表达降低,间质性标志Fibronectin、Vimentin表达升高,同时抑癌因子PTEN表达下降。[结论]过表达ZNF488可通过诱导鼻咽癌细胞HONE1发生上皮间质转变及下调抑癌基因PTEN,促进细胞的增殖及迁移侵袭能力。 [Objective] To investigate the effect of ZNF488 gene on the biological behavior of nasopharyngeal carcinoma cell HONE1. [Methods] Retroviral plasmids pMSCV and pMSCV-ZNF488 carrying empty vector and human ZNF488 gene were constructed and transfected into 293FT cells by calcium phosphate method. The virus fluid was collected and infected into nasopharyngeal carcinoma cell line HONE1. After puromycin selection and Western blotting, Blot identification, the establishment of stable expression of HONE1-pMSCV-ZNF488 ZNF488 and empty vector HONE1-pMSCV cell lines. The effect of ZNF488 overexpression on the growth and proliferation of HONE1 cells was detected by MTT assay and plate clone formation assay. The effect of ZNF488 on cell cycle proliferation index was also detected by flow cytometry. Scratch assay and Transwell invasion assay observe the effect of ZNF488 on HONE1 migration and invasion. Western Blot was used to detect the expression of epithelial-mesenchymal transition (EMT) related protein and tumor suppressor gene PTEN. [Results] The HONE1-pMSCV-ZNF488 cell line stably expressing ZNF488 was successfully established. MTT assay and plate clone formation assay showed that the proliferation and clonogenic capacity of HONE1-pMSCV-ZNF488 cells compared with the empty vector group HONE1-pMSCV cells Enhanced. Flow cytometry showed that the percentage of S phase of over-expressing ZNF488 cells was higher than that of empty vector group, and the proliferation index increased. Scratch experiment, Transwell in vitro invasion experiments showed that ZNF488 can significantly increase cell migration and invasion ability. Western Blot showed that the expression of E-Cadherin and α-Catenin decreased, while the expression of Fibronectin and Vimentin increased, while the expression of tumor suppressor PTEN decreased. [Conclusion] Overexpression of ZNF488 can induce the epithelial-mesenchymal transition of HONE1 cells and down-regulate the tumor suppressor gene PTEN to promote cell proliferation and migration.