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目的:研究贵州世居少数民族-黔西、威宁彝族人群IL-10基因启动子区-819位点(IL10-819)和趋化因子受体5(chemokine receptor 5,CCR5)基因启动子区-59029位点(CCR5-59029)与HBV易感性的相关性.方法:对研究人群采用序列特异性引物-聚合酶链式反应(PCR-SSP)方法分析IL-10基因启动子区-819位点多态性和限制性片段长度多态性-聚合酶链式反应(PCR-RFLP)方法分析CCR5基因启动子区-59029位点.结果:IL10-819基因型频率在贵州黔西彝族感染组与非感染组中的分布差异有统计学意义(P<0.05),在贵州威宁彝族感染组与非感染组中的分布差异无统计学意义;CCR5-59029基因威宁彝族感染组与非感染组中的分布差异有统计学意义(P<0.05),在贵州黔西彝族感染组与非感染组中的分布差异无统计学意义.结论:IL10-819基因和CCR5-59029基因多态性与彝族乙肝感染情况可能存在相关性,IL10-819基因和CCR5-59029基因多态性可能是乙肝易感性的因素之一.
OBJECTIVE: To study the promoter region of IL10-819 and chemokine receptor 5 (CCR5) genes in IL-10 gene promoter region of Guizhou, a minor nationality in Guizhou and Weining Yi ethnic groups. -59029 (CCR5-59029) and HBV susceptibility.Methods: The study population was analyzed by sequence-specific primer-polymerase chain reaction (PCR-SSP) Point polymorphism and restriction fragment length polymorphism-polymerase chain reaction (PCR-RFLP) were used to analyze the -59029 locus of CCR5 gene.Results: The frequency of IL10-819 genotype was significantly higher in Guizhou Yi people than in Guizhou province And non-infected group (P <0.05). There was no significant difference in the distribution of Weining Yi infection group and non-infected group in Guizhou. The distribution of CCR5-59029 Yi-wei Yi infection group was significantly higher than that of non-infected group (P <0.05), but there was no significant difference in the distribution of infection and non-infection among Yi people in Qianxi, Guizhou.Conclusion: IL10-819 gene and CCR5-59029 gene polymorphism were associated with Yi Hepatitis B infection may be related to the situation, IL10-819 gene and CCR5-59029 gene polymorphism may Hepatitis B is one of the factors susceptibility.