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目的 研究快速老化模型小鼠 (senescenceacceleratedmice ,SAM )学习记忆能力及其大脑皮层、海马和下丘脑单胺递质含量的增龄性变化及它们之间的关系。方法 分别采用跳台实验和穿梭箱实验测定SAM的被动和主动回避反应能力 ,采用高效液相色谱电化学检测法测定脑内单胺递质的含量。 结果 2月龄快速老化亚系SAM prone/ 8(SAMP8)的被动和主动回避反应能力已较同龄抗快速老化亚系SAM resistance/ 1(SAMR1)明显降低 ,且其主动回避反应能力随增龄进一步降低。同时 ,SAMP8大脑皮层、海马及下丘脑内单胺递质水平多明显高于同龄SAMR1,且随增龄明显增高。 结论 SAMP8学习记忆能力的衰退可能与其相关脑区单胺递质的变化密切相关。
Objective To study the learning and memory abilities of senescence accelerated mice (SAM) and the age-related changes of monoamine neurotransmitters in the cerebral cortex, hippocampus and hypothalamus of aged mice and their relationship. Methods The passive and active avoidance responses of SAM were determined by the platform test and the shuttle box experiment, respectively. The content of monoamine neurotransmitters in the brain was determined by high performance liquid chromatography electrochemical detection. Results The passive and active avoidance responses of SAM prone / 8 (SAMP8), a rapid aging sub-line of 2-month old, were significantly lower than that of the SAM resistance / 1 (SAMR1) anti-fast aging sub-line of the same age group, and their active avoidance ability further increased with age reduce. At the same time, the levels of monoamine neurotransmitters in cerebral cortex, hippocampus and hypothalamus of SAMP8 were significantly higher than SAMR1 of the same age, and increased significantly with age. Conclusions The decline of learning and memory abilities of SAMP8 may be closely related to the changes of monoamine neurotransmitters in brain regions associated with SAMP8.