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郎格罕氏细胞(LC)系起源于骨髓,表面携带Fc-IgG和C3受体,合成与表达I_a抗原,主要分布在表皮基底层上方的一种树枝状免疫前哨细胞。在众多显示该细胞的组化及免疫学方法中以ATP_(ase)染色应用最为广泛。该方法经济,操作简便,特异性高。笔者根据Juhlin和Hanau等人方法加以改良而建立的ATP_(ase)染色具有2个突出优点:(1)应用显示ATP_(ase)活性的单一技术可以同时进行光、电镜对比研究,而细胞结构基本保存完好。(2)解决半薄切片上Lc定位问题,避免电镜观察盲目性。实验方法:用2.5％戊巴比妥钠,以25mg/kg剂量腹腔注射,麻醉豚鼠。背部剃、脱毛后,用3mm直径Biopsy Punch活检皮肤,标本置EDTA液内孵育1.5～2小时,37℃水浴。实验者在解剖显微镜下用
Langerhans cell (LC) is a dendritic immune outpost cell originating from the bone marrow, carrying Fc-IgG and C3 receptors on the surface and synthesizing and expressing I-a antigen, mainly distributed above the basal layer of the epidermis. Among the many histochemical and immunological methods that show this cell, ATPase staining is the most widely used. The method is economical, easy to operate and highly specific. ATPase staining developed by the author according to the method of Juhlin and Hanau et al. Has two outstanding advantages: (1) A single technique for displaying ATPase activity can simultaneously perform light and electron microscopic studies, while the basic cell structure well-preserved. (2) to solve the Lc positioning problem on the semi-thin slice to avoid blind observation by electron microscopy. Experimental Methods: Guinea pigs were anesthetized with 2.5% pentobarbital sodium and 25 mg / kg ip. Back shaved, hair removal, Biopsy Punch with 3mm diameter biopsy of the skin specimens EDTA incubated 1.5 to 2 hours, 37 ℃ water bath. The experimenter uses a dissecting microscope