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目的:探讨中国HIV-1 B’/C亚型感染者对自身病毒中和作用与疾病进展的关系。方法:将24例HIV-1 B’/C感染者自身原代病毒与同期和6个月自身血浆作用后,感染正常PBMC,培养7天测定p24抗原浓度,以正常人血浆加病毒悬液为对照。以抑制50%对照孔p24浓度的血浆最高稀释倍数的倒数计算中和抗体滴度,中和抗体滴度≥8倍为具有中和作用。结果:在同期血浆中和自身病毒试验中,3例缓慢进展者(SP)均具有中和作用,HIV组仅4例(4/21)具有中和作用,SP组中和抗体滴度明显高于HIV组。在6个月血浆中和自身病毒试验中,SP组中和抗体滴度明显增加,HIV组12例具有中和作用,SP组中和抗体滴度明显高于HIV组。中和抗体滴度与病毒载量呈明显的负相关。结论:疾病缓慢进展的HIV-1 B’/C亚型感染者对自身病毒中和作用明显高于HIV组,提示中和抗体在延缓疾病进程中发挥重要作用。
Objective: To investigate the relationship between self-virus neutralization and disease progression in HIV-1 B ’/ C subtype in China. Methods: 24 cases of HIV-1 B ’/ C infected primary self-virus and the same period and 6 months of plasma after infection with normal PBMC, cultured 7 days determination of p24 antigen concentration in normal plasma plus virus suspension was Control. Neutralizing antibody titers were calculated as the reciprocal of the highest dilution of plasma that inhibited 50% of control well p24 concentrations and neutralizing antibody titers> 8 times as neutralizing. Results: Neutralization was observed in 3 patients with slow progression (SP) during the same period of plasma neutralization and self-virus test. Only 4 patients (4/21) in the HIV group neutralized and the neutralizing antibody titer in SP group was significantly higher In the HIV group. In the 6-month plasma and self-virus test, the titer of neutralizing antibody in SP group increased significantly. In HIV group, 12 patients had neutralization and neutralizing antibody titer in SP group was significantly higher than that in HIV group. Neutralizing antibody titers showed a significant negative correlation with viral load. CONCLUSIONS: HIV-1 B ’/ C subtype infected with slow disease has significantly higher self-virus neutralization effect than HIV group, suggesting that neutralizing antibody plays an important role in delaying disease progression.