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目的探讨维药榅桲多糖对3T3-L1小鼠胚胎成纤维细胞增殖及胰岛素抵抗的作用。方法培养3T3-L1脂肪细胞,通过噻唑蓝比色(MTT)法观察不同浓度榅桲多糖对细胞增殖的影响;通过油红O染色法并测定甘油三酯(TG)的含量,评价前脂肪细胞是否诱导为成熟脂肪细胞;采用地塞米松为诱导剂建立3T3-L1胰岛素抵抗细胞模型,分别给予不同浓度榅桲多糖进行干预后,以葡萄糖氧化酶法检测培养液中葡萄糖残留量。结果与正常对照组比较,榅桲多糖能明显抑制3T3-L1脂肪细胞增殖;油红O染色法判定前脂肪细胞成功诱导为成熟脂肪细胞,TG含量测定结果表明,与正常对照组相比成熟3T3-L1细胞中脂质含量增多(P<0.01);在3T3-L1胰岛素抵抗细胞模型中,与正常对照相比榅桲多糖可明显增加葡萄糖利用率(P<0.05)。结论榅桲多糖具有抑制脂肪细胞增殖的作用并可明显改善地塞米松诱导的胰岛素抵抗。
Objective To investigate the effect of polysaccharide of xylene xanthium on proliferation and insulin resistance of 3T3-L1 mouse embryonic fibroblasts. Methods 3T3-L1 adipocytes were cultured and the effects of polysaccharides from different concentrations of polysaccharides were observed by MTT assay. The levels of triglyceride (TG) were determined by oil red O staining, Whether induced to mature adipocytes; Dexamethasone as an inducer to establish 3T3-L1 insulin resistance cell model, respectively, given different concentrations of polysaccharides polysaccharide intervention, glucose oxidase method to detect glucose residues in the culture medium. Results Compared with the normal control group, the polysaccharide of Smilax mongholicus significantly inhibited the proliferation of 3T3-L1 adipocytes. The preadipocytes were successfully induced to mature adipocytes by Oil Red O staining. The results of TG assay showed that compared with the normal control group, mature 3T3 (P <0.01). In 3T3-L1 insulin resistance cell model, polysaccharides significantly increased the glucose utilization rate (P <0.05) compared with the normal control. Conclusion Polysaccharide polysaccharide can inhibit the proliferation of adipocytes and can significantly improve dexamethasone-induced insulin resistance.