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Aim: To identify the metastasis suppressor genes for prostate cancer. Methods: A copy of human chromosomeswas introduced into the highly metastatic Dunning R-3327 rat prostate cancer cells by the use of microcell-mediatedchromosome transfer. Relationships between the size of human chromosomes introduced into microcell hybrid clonesand the number of lung metastases produced by the clones were analyzed to determine which part of human chromo-somes contained the metastasis suppressor gene (s) for prostate cancer. To determine portions of human chromosomesintroduced, G-banding chromosomal analysis, fluorescence in sim hybridization analysis, and polymerase chain reac-tion analysis were performed. Results: Each of microcell hybrid clones containing human chromosomes 7, 8, 10,11, 12, or 17 showed decreased ability to metastasize to the lung without any loss of tumorigenicity. This demonstratesthat these human chromosomes contain metastasis suppressor genes for prostate cancer. Spontaneous deletion of portions
Aim: To identify the metastasis suppressor genes for prostate cancer. Methods: A copy of human chromosomeswas introduced into the highly metastatic Dunning R-3327 rat prostate cancer cells by the use of microcell-mediatedchromosome transfer. Relationships between the size of human chromosomes introduced into Microcell hybrid clonesand the number of lung metastases produced by the clones were analyzed to determine which part of human chromo-somes contained the metastasis suppressor gene (s) for prostate cancer. To determine parts of human chromosomesintroduced, G-banding chromosomal analysis, fluorescence in Sim hybridization analysis, and polymerase chain reac-tion analysis were performed. Each of the microcell hybrid clones containing human chromosomes 7, 8, 10, 11, 12, or 17 showed decreased ability to metastasize to the lung without any loss of tumorigenicity. This demonstratesthat these human chromosomes contain metastasis suppressor genes for prostate cancer. Spontaneous de Letion of portions