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目的:建立测定辣椒面中刚果红染料含量的超高效液相色谱方法。方法:采用氨水-乙醇(30∶70,V/V)提取剂,超声加热辅助提取3次,合并提取液、浓缩后过HLB固相萃取小柱,以5 mL 1%甲酸-甲醇溶液淋洗、5 mL 10%氨水-甲醇溶液洗脱,收集洗脱液、氮吹浓缩后定容测定。使用BEH C18色谱柱(50 mm×2.1 mm,1.7 μm);流动相:A为乙腈,B为10 mmol/L乙酸铵溶液,梯度洗脱;流速0.4 mL/min;检测波长498 nm;柱温40℃。结果:回收率为72%~96%,方法检出限为0.0290mg/kg,相对标准偏差为2.16%~5.97%(n=6),线性方程相关系数达到0.9999。结论:本方法简便、快速、准确、灵敏,适用辣椒面中刚果红染料含量的测定。
Objective: To establish a method for the determination of Congo red dye in capsicum by ultra performance liquid chromatography. Methods: Aqueous ammonia-ethanol (30:70, V / V) extractant was used to extract three times by ultrasonic heating. The extracts were combined and concentrated on a HLB solid-phase extraction cartridge and eluted with 5 mL of 1% formic acid- , 5 mL 10% aqueous ammonia - methanol solution was eluted, the eluent was collected, nitrogen concentration after the determination of the volume. Using a BEH C18 column (50 mm × 2.1 mm, 1.7 μm); mobile phase: A was acetonitrile, B was 10 mmol / L ammonium acetate solution, gradient elution; flow rate was 0.4 mL / min; detection wavelength was 498 nm; 40 ° C. Results: The recoveries ranged from 72% to 96%. The detection limits were 0.0290 mg / kg and the relative standard deviations were 2.16% -5.97% (n = 6). The correlation coefficient of linear equation reached 0.9999. Conclusion: The method is simple, rapid, accurate and sensitive and suitable for the determination of congo red dye content in red pepper.