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目的:检测喉癌中基质金属蛋白酶11(MMP11)的表达,探讨MMP11的表达与喉癌浸润和转移的关系。方法:采用免疫组织化学法检测63例喉癌组织和相应的癌旁正常组织MMP11蛋白的表达,运用RT-PCR技术检测22例喉癌和相应的癌旁正常组织冻存标本中MMP11 mRNA水平的表达。结果:采用免疫组织化学法检测喉癌和癌旁正常组织MMP11的表达,癌组织MMP11的表达阳性率明显高于癌旁正常组织(P<0.01)。运用RT-PCR方法检测喉癌和癌旁正常组织MMP11 mRNA的表达,癌组织MMP11 mRNA的表达阳性率明显高于癌旁正常组织(P<0.01)。MMP11蛋白表达在T3+T4组高于T1+T2组(P<0.01),淋巴结转移组高于非淋巴结转移组(P<0.05)。MMP11 mRNA表达在T3+T4组高于T1+T2组(P<0.05),淋巴结转移组高于非淋巴结转移组(P<0.05)。MMP11蛋白及MMP11 mRNA的表达与肿瘤原发部位和分化程度无明显相关性(P>0.05)。结论:MMP11的表达与喉癌浸润和转移密切相关,可作为预测喉癌浸润和转移潜能的参考指标之一。
Objective: To detect the expression of matrix metalloproteinase 11 (MMP11) in laryngeal carcinoma and to explore the relationship between the expression of MMP11 and the invasion and metastasis of laryngeal carcinoma. Methods: The expression of MMP11 protein in 63 cases of laryngeal carcinoma and adjacent normal tissues was detected by immunohistochemistry. The expression of MMP-1 mRNA in 22 cases of laryngeal carcinoma and adjacent normal tissues was detected by RT-PCR. expression. Results: The positive expression rate of MMP11 in laryngeal carcinoma and adjacent normal tissue was significantly higher than that in adjacent normal tissues (P <0.01) by immunohistochemistry. The expression of MMP11 mRNA in laryngeal carcinoma and adjacent normal tissues was detected by RT-PCR. The positive rate of MMP11 mRNA in cancer tissues was significantly higher than that in adjacent normal tissues (P <0.01). The expression of MMP11 protein in T3 + T4 group was higher than that in T1 + T2 group (P <0.01). The expression of MMP11 protein in lymph node metastasis group was higher than that in non - lymph node metastasis group (P <0.05). The expression of MMP11 mRNA in T3 + T4 group was higher than that in T1 + T2 group (P <0.05). The expression of MMP11 mRNA in lymph node metastasis group was higher than that in non - lymph node metastasis group (P <0.05). The expression of MMP11 protein and MMP11 mRNA had no significant correlation with the primary tumor location and differentiation (P> 0.05). Conclusion: The expression of MMP11 is closely related to the invasion and metastasis of laryngeal carcinoma, which may be used as a reference index for predicting the invasion and metastasis of laryngeal carcinoma.