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采用不同成熟度的花生胚轴为外植体进行体细胞胚诱导及植株再生研究,结果表明,成熟胚轴在高浓度2,4-D的MS培养基中,经过30d左右的培养,可直接诱导产生出大量的体细胞胚,含40mgL~-12,4-D的培养基中体细胞胚的诱导率达100%,平均每个外植体产生11.58个体细胞胚.体细胞胚的继代培养需降低2,4-D的浓度(1-20mgL~-1).未成熟胚轴的体细胞胚诱导及继代培养的2,4-D浓度宜为10mgL~-1.将诱导的体细胞胚转接到合5-10mgL~-1BA的MS培养基中,体细胞胚能够萌发再生成无根小植株,将其转接到生根培养基中可获得完整小植株.
The embryos of peanut with different maturity were used as explants to induce somatic embryogenesis and plant regeneration. The results showed that the mature hypocotyls could be directly cultured in high concentration of 2,4-D MS medium after about 30 days Induced to produce a large number of somatic embryos, with 40mgL ~ -12,4-D medium somatic embryos induction rate of 100%, an average of 11.58 somatic embryos per explant. Subcultures of somatic embryos need to be reduced 2,4-D concentration (1-20mgL ~ -1). The 2,4-D concentration of immature embryo somatic embryos induced and subculture should be 10 mgL ~ (-1). The induced somatic embryos were transferred to MS medium supplemented with 5-10 mg L ~ -1 BA. Somatic embryos were able to germinate and regenerate into rootless plantlets, and then transferred to rooting medium to obtain intact plantlets.