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生物化学物质在红外范围内具有特有的吸收光谱。作者认为可利用红外ATR分光法对渗出液进行测定。 ATR属于反射分光法,在采用内部反射分光时,使入射光通过高于被检液折射率的棱镜,从而观测被检液与棱镜界面处的反射光。保持入射角大于临界角,使之在界面处发生全反射。然而,当被检液吸收光时,就不会发生全反射,部分入射光的能量丧失于被检液中,反射率也相应减少。这一状态称为衰减全反射,采用这种反射测定法的分光分析,称为内部反射分光法或ATR分光法。ATR法的特点是,只测定被检液表层的吸收,其可测深度最多与所采用的波长相
Biochemicals have unique absorption spectra in the infrared range. The authors believe that the use of infrared ATR spectrophotometry exudate determination. ATR belongs to the reflection spectroscopy method. When using internal reflection spectroscopy, the incident light is passed through a prism whose refractive index is higher than that of the test liquid to observe the reflected light at the interface between the liquid to be tested and the prism. Keep the angle of incidence above the critical angle so that total reflection occurs at the interface. However, when the test liquid absorbs light, total reflection does not occur, and part of incident light energy is lost in the test liquid and the reflectance is also reduced accordingly. This condition is known as attenuated total reflection. Spectroscopic analysis using this reflectometry is called internal reflection spectroscopy or ATR spectroscopy. The ATR method is characterized by measuring only the absorption of the surface layer of the liquid to be tested, which can measure the depth up to the wavelength used