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以50％的聚乙二醇(分子量1,000)诱导培养的Eca-109细胞系(人食管癌细胞系),和CHO细胞系(中国地鼠卵巢细胞系)融合。结果表明:聚乙二醇处理后10天内,融合率逐渐下降,大核细胞日渐增多,而多核细胞日趋减少。由Eca-109,CHO同种细胞间融合形成的双核细胞中,仍保持亲本细胞的形态;而异种双核细胞可显示某一亲本之形态,或呈介于二者之间的圆形、不规则形;三核、多核细胞则呈不规则形。组织化学检查表明,融合细胞中(无论是同核体或是异核体)乳酸脱氢酶、谷氮酸脱氢酶、苹果酸脱氢酶反应,明显较单个亲本细胞强。这可能是融合细胞中各种生理状态改变,导致对ATP需求量增加,从而引起相应酶活性升高。融合细胞中各亲本基因共同表达,杂交酶的产生也可能导致较单个亲本细胞强的酶反应。异核体中非特异性酯酶活性居于二亲本活性水平之间,即高于CHO,低于Eca-109。
The cultured Eca-109 cell line (human esophageal cancer cell line) was induced with 50% polyethylene glycol (molecular weight 1,000) and fused with a CHO cell line (Chinese hamster ovary cell line). The results showed that within 10 days after PEG treatment, the fusion rate gradually decreased, the number of large nuclear cells increased, and the number of multinucleated cells decreased. In the binucleated cells formed by the fusion of Eca-109 and CHO cells, the morphology of the parental cells is still maintained. However, the heterologous dinuclear cells can show the morphology of a certain parent or a round, irregular Shaped; trinuclear, multinucleated cells were irregular shape. Histochemical examination showed that the lactate dehydrogenase, glutamate dehydrogenase, and malate dehydrogenase reactions in the fused cells (either oncogene or heterokaryon) were significantly stronger than single parental cells. This may be due to changes in various physiological states in the fusion cells, resulting in an increased demand for ATP, resulting in an increase in the corresponding enzyme activity. The expression of the respective parental genes in the fusion cells is also likely to result in a stronger enzyme reaction than the single parental cell. Nonspecific esterase activity in heterokaryons resided between the levels of the two parents, higher than CHO and lower than Eca-109.