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目的:探讨硫辛酸(Lipoic acid,LA)对卵巢切除(ovariectomized,OVX)小鼠学习记忆的影响。方法:2月龄雌性昆明小鼠随机分为4组:假手术(Sham)组、OVX组、OVX+LA组、OVX+LA+左旋硝基精氨酸甲酯(L-NAME)组。建立卵巢切除模型并给药,Morris水迷宫检测各组小鼠学习记忆能力。结果:前4天定位巡航实验中,OVX组与Sham组相比,逃避潜伏期明显延长(P<0.05),第5天撤除平台后,OVX组在目的象限游泳距离及时间百分比较Sham组明显缩短(P<0.05);OVX+LA组与OVX组相比,逃避潜伏期明显缩短(P<0.05),撤除平台后,在目的象限游泳距离及时间百分比明显延长(P<0.05);OVX+LA+L-NAME组与Sham组相比,逃避潜伏期明显延长(P<0.05),撤除平台后,在目的象限游泳距离及时间百分比明显缩短(P<0.05)。结论:OVX损伤小鼠的学习记忆,LA可改善这种损伤,内皮型一氧化氮合酶(e NOS)抑制剂L-NAME可削弱LA的这种保护作用。
Objective: To investigate the effect of lipoic acid (LA) on learning and memory in ovariectomized (OVX) mice. Methods: Female Kunming mice of 2 months old were randomly divided into 4 groups: sham group, OVX group, OVX + LA group, OVX + LA + L-NAME group. The model of ovariectomy was established and administered. The Morris water maze was used to detect the learning and memory ability of mice in each group. Results: In the first four days of positioning cruise test, the evasion latency of OVX group was significantly longer than that of Sham group (P <0.05). After the platform was removed on the fifth day, the distance and time percentage of swimming in the destination quadrant of OVX group was significantly shorter than Sham group (P <0.05). Compared with OVX group, the escape latency of OVX + LA group was significantly shorter than that of OVX group (P <0.05). After removing the platform, the swimming distance and time percentage in the destination quadrant were significantly prolonged Compared with Sham group, the L-NAME group had a significantly longer escape latency (P <0.05). After removing the platform, the swimming distance and time percentage in the destination quadrant were significantly shorter (P <0.05). Conclusion: Learning and memory of OVX injured mice, LA can improve this injury. L-NAME, an inhibitor of endothelial nitric oxide synthase (eNOS), can attenuate this protective effect of LA.