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目的研究磷酸烯醇式丙酮酸羧激酶(PCK1)启动子232基因多态性与不同亚型缺血性脑卒中的关系。方法 165例缺血性脑卒中患者,采用CISS分型法分为动脉粥样硬化血栓形成亚组(AT)、急性穿支小动脉闭塞亚组(APSAO)和心源性栓塞亚组(CE),并选取健康成人90例作为对照组,采用聚合酶链反应-限制性片段长度多态性方法,进行PCK1启动子-232基因多态性分析,比较各组间基因型分布及等位基因频率。结果 (1)PCK1启动子232突变纯合子GG基因型及突变G等位基因频率,在AT、APSAO亚组显著高于对照组(P<0.05),而CE组突变基因型频率及突变等位基因频率与对照组比较,差异无统计学意义(P>0.05)。(2)收缩压、低密度脂蛋白胆固醇(LDL-C)、高血压史、糖尿病史、PCK1启动子232基因多态性(P=0.005)是CIS的危险因素。结论 PCK1启动子232基因多态性可能与CIS中AT、APSAO亚型相关。
Objective To investigate the relationship between the polymorphism of phosphoenolpyruvate carboxykinase (PCK1) promoter 232 and ischemic stroke in different subtypes. Methods A total of 165 patients with ischemic stroke were divided into atherothrombosis subgroup (AT), APSAO group and cardioembolism group (CE) by CISS classification. , And 90 healthy adults were selected as control group. The polymorphism of PCK1 promoter-232 gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype distribution and allele frequency . Results (1) The frequencies of GG genotype and G allele in PCK1 promoter 232 mutant were significantly higher in AT and APSAO subgroups than those in control group (P <0.05), while those in CE group were significantly higher than those in control group There was no significant difference in gene frequency between the two groups (P> 0.05). (2) The systolic blood pressure, low density lipoprotein cholesterol (LDL-C), history of hypertension, history of diabetes, PCK1 promoter 232 gene polymorphism (P = 0.005) were the risk factors of CIS. Conclusion The polymorphism of PCK1 promoter 232 gene may be related to the AT and APSAO subtypes in CIS.