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目的研究转移相关基因Notch1和VEGF在15例正常卵巢组织、32例卵巢良性肿瘤组织和48例卵巢恶性癌组织中的表达及相关性研究,旨在为卵巢癌的病理诊断及发病机制的探讨提供理论依据。方法采用免疫组化SP法检测各组Notch1、VEGF的表达情况和微血管密度(MVD),MVD采用CD34标记。并通过统计学方法分析其表达情况与临床病理特征的关系及相互间的关系。结果 Notch1在正常卵巢组织、卵巢良性肿瘤组织和卵巢恶性癌组织中的阳性表达率分别为13.33%(2/15)、31.25%(10/32)和81.25%(39/48);VEGF在正常卵巢组织、卵巢良性肿瘤组织和卵巢恶性癌组织中的阳性表达率分别为20%(3/15)、47.0%(15/32)和83.0%(40/48);CD34主要表达于各种卵巢组织的血管内皮细胞,MVD值在有淋巴结转移的卵巢癌组织中明显高于无淋巴结转移者(P<0.05)。通过Spearman相关性分析,Notch1和VEGF在卵巢恶性癌组织中呈现显著正相关(P<0.05)。结论 Notch1、VEGF是鉴别卵巢良恶性肿瘤的指标之一,检测淋巴结转移者中的MVD值对卵巢癌的恶性程度判断有帮助。
Objective To study the expression and correlation of metastasis-related genes Notch1 and VEGF in 15 cases of normal ovarian tissue, 32 cases of ovarian benign tumor and 48 cases of ovarian cancer, and to provide a theoretical basis for the pathological diagnosis and pathogenesis of ovarian cancer Theoretical basis. Methods Immunohistochemical SP method was used to detect the expression of Notch1 and VEGF and the microvessel density (MVD) in each group. MVD was labeled with CD34. And through statistical methods to analyze the relationship between their expression and clinicopathological features and the relationship between them. Results The positive rates of Notch1 in normal ovarian tissue, ovarian benign tumor and ovarian cancer were 13.33% (2/15), 31.25% (10/32) and 81.25% (39/48) The positive expression rates of CD34 in ovarian tissues, benign ovarian tumor tissues and ovarian cancer tissues were 20% (3/15), 47.0% (15/32) and 83.0% (40/48), respectively. Tissue vascular endothelial cells, MVD values in lymph node metastasis of ovarian cancer was significantly higher than those without lymph node metastasis (P <0.05). Spearman correlation analysis showed that there was a significant positive correlation between Notch1 and VEGF in ovarian cancer (P <0.05). Conclusions Notch1 and VEGF are one of the indexes for differentiating benign and malignant ovarian tumors. Detecting the MVD in lymph node metastasis may be helpful in judging the malignancy of ovarian cancer.