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目的探讨槐定碱对大鼠肝硬化形成及内毒素水平的干预作用。方法将75只大鼠随机分为空白对照组、肝硬化模型组、槐定碱低、中、高剂量干预组。肝硬化模型组用四氯化碳复合法复制肝硬化模型,槐定碱低、中、高剂量干预组在此基础上于皮下注射四氯化碳后1h分别腹腔注射槐定碱36、、10mg.kg-1,每周2次,注射8周。实验8周后观察肝脏的病理变化并采用显色基质鲎试剂试管法测定大鼠血浆内毒素水平。结果病理观察发现肝硬化模型组肝组织有假小叶形成,而三个剂量槐定碱干预组的肝组织均无假小叶形成,纤维化程度较低。五组大鼠血浆内毒素浓度的总体均数不相等,每两组大鼠血液内毒素浓度比较差别均有统计学意义(P<0.05)。结论槐定碱可以通过抗内毒素而延缓肝硬化的形成,不同剂量槐定碱干预效果不同,具有一定的剂量-效应关系。
Objective To investigate the effects of sophoridine on hepatic cirrhosis and endotoxin levels in rats. Methods 75 rats were randomly divided into blank control group, liver cirrhosis model group and sophoridine low, medium and high dose intervention groups. Liver cirrhosis model group was treated with carbon tetrachloride complex method to replicate liver cirrhosis models. Sophoridine low, medium and high dose intervention groups were given intraperitoneal sophoridine 36, 1 mg kg-1, twice a week for 8 weeks. Eight weeks after the experiment, the pathological changes of the liver were observed and the level of plasma endotoxin in rats was determined by colorimetric matrix 鲎 reagent tube method. Results Pathological observation showed that there was pseudolobule formation in the liver of the liver cirrhosis model group. No pseudolobule was formed in the liver tissue of the three dose sophoridine groups, and the degree of fibrosis was lower. The mean total plasma endotoxin concentrations in five groups of rats were not equal. The blood endotoxin concentrations in each group were significantly different (P <0.05). Conclusion Sophoridine can delay the formation of hepatic cirrhosis through anti-endotoxin. Different doses of sophoridine interfere with each other and have a dose-effect relationship.