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背景:去甲肾上腺素能药物苯丙胺可提高脑缺血后动物运动功能的恢复。氯丙米嗪抑制5-羟色胺和去甲肾上腺素再摄取,提高脑内去甲肾上腺素和5-羟色胺水平。目的:观察氯丙米嗪对局灶性脑缺血损伤后大鼠运动功能的作用。设计:随机分组设计,对照动物实验。单位:解放军第四军医大学航空临床医学教研室神经精神病学组。材料:实验在第四军医大学航空航天医学系形态学实验室完成。选择SD大鼠24只随机分为假手术组、缺血组、缺血用药组,每组8只。缺血用药组动物于缺血后24h经口灌注2.5g/L氯丙米嗪溶液(10mg/kg),1次/d。假手术组、缺血组动物经口灌注相应容量的蒸馏水。方法:采用插线法制作大脑中动脉缺血/再灌注大鼠模型,造模成功后进行①网屏握持试验:将网屏水平放置,将鼠放于其上,然后缓慢地将其一端抬高,在2s内将此网屏翻转成125°位,并保持于该位,记录大白鼠在网屏上握持的时间。②胶布撕脱试验:将0.5cm2的医用胶布粘于大鼠两前爪腹侧面后,送其入观察箱中,记录其撕去胶布所使用的时间。各组实验动物在术后1,3,7,14,28d为观察时间点。主要观察指标:大鼠局灶性脑缺血后网屏握持时间和胶布撕脱时间。结果:①与假手术组比较,缺血组和缺血用药组大鼠网屏肌力测试时间缩短,差异非常显著眼以术后3d为例,假手术组、缺血组、缺血用药组分别为(54±4),(20±5),(21±4)s,P<0.01演。缺血用药组与缺血组比较网屏握持时间延长,差异显著眼以术后28d为例,缺血组、缺血用药组分别为(51±5),(54±5)s,P<0.05演。②缺血组和缺血用药组大鼠胶布撕脱时间均比假手术组长,差异非常显著眼以术后3d为例,假手术组、缺血组、缺血用药组分别为(47±9),(188±20),(172±22)s,P<0.01演。缺血用药组比缺血组大鼠胶布撕脱时间略短,差异无显著性(P>0.05)。结论:氯丙米嗪对局灶性脑缺血后肌力恢复作用明显,而对感觉和精细运动功能作用不明显,此与偏瘫后肢体精细功能恢复差的特点相符。
BACKGROUND: Norepinephrine amphetamine enhances the recovery of motor function after cerebral ischemia. Chlorpromazine inhibits serotonin and norepinephrine reuptake and increases brain norepinephrine and serotonin levels. Objective: To observe the effect of chlorpromazine on motor function after focal cerebral ischemia in rats. Design: randomized block design, controlled animal experiments. Unit: Department of Neurology and Psychiatry, PLA Aviation Medical College, Fourth Military Medical University. Materials: Experiments were performed at the Morphology Laboratory, Department of Aeronautics and Astronautics, Fourth Military Medical University. Twenty-four SD rats were randomly divided into sham-operated group, ischemia group and ischemia-treated group, with 8 rats in each group. Animals in ischemic group were orally injected 2.5g / L of clomipramine solution (10mg / kg) once a day for 24 hours after ischemia. Sham operation group, ischemia group animals were perfused with the corresponding volume of distilled water. Methods: The middle cerebral artery occlusion (MCAO) / reperfusion model was made by the plug-in method. After the model was successfully established, the screen holding test was carried out: the screen was placed horizontally, the mouse was placed on it, and then slowly one end Raise the screen in 2s to flip this screen to 125 °, and keep in that position, record the mouse holding time on the screen. ② tape avulsion test: The 0.5cm2 medical tape affixed to the ventral side of the rat two forepaws, send it into the observation box, record the time it takes to tear off the tape. Each group of experimental animals at 1, 3, 7, 14, 28d after surgery for the observation time point. MAIN OUTCOME MEASURES: Screen holding time and tape avulsion time after focal cerebral ischemia in rats. Results: ① Compared with the sham operation group, the time of screen muscle strength test was shortened in ischemic group and ischemic group, the difference was significant. Taking 3d postoperatively as an example, sham operation group, ischemia group, ischemia administration group (54 ± 4), (20 ± 5), (21 ± 4) s, P <0.01 respectively. In the ischemic group, the holding time of the screen was longer than that of the ischemic group. The difference was significant at 28 days after operation. The ischemic and ischemic groups were (51 ± 5), (54 ± 5) s, P <0.05 performance. ② The ischemic group and ischemic group were longer than the sham operation group, the difference was significant (P <0.05). The postoperative 3d was taken as an example. The sham group, ischemic group and ischemic group were (47 ± 9), (188 ± 20), (172 ± 22) s, P <0.01. The ischemic group than the ischemic group rats tape slightly shorter avulsion, the difference was not significant (P> 0.05). CONCLUSION: Chlorpromazine has a significant effect on the recovery of muscle strength after focal cerebral ischemia but has no obvious effect on sensory and motor function. This is consistent with the poor recovery of fine function after hemiplegia.