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目的 :研究人 β胰岛细胞Fas的表达及细胞因子诱导Fas表达量的增加在 β胰岛细胞杀伤中的作用。 方法 :用FACS检测IFN γ和TNF α作用于人β胰岛细胞株 (NIT)前后Fas表达水平及sFasL和抗Fas介导细胞凋亡的变化。 结果 :NIT表面无Fas表达 ,IFN γ和TNF α可促进其Fas表达增加 ,以及对sFasL和anti Fas介导细胞毒敏感性增加。结论 :细胞因子诱导 β胰岛细胞表面Fas的表达可能在I型糖尿病 (IDDM)的病理损伤中起重要作用
AIM: To investigate the role of Fas in human beta islet cells and the increase of Fas expression induced by cytokines in beta islet cell killing. Methods: FACS was used to detect the expression of Fas and the effect of sFasL and anti-Fas-mediated apoptosis of IFN-γ and TNF-alpha on human beta islet cell line (NIT). RESULTS: No Fas expression was observed on the surface of NIT. IFNγ and TNFα promoted the increase of Fas expression and increased cytotoxicity of sFasL and anti Fas. CONCLUSIONS: Cytokine-induced Fas expression on beta islet cells may play an important role in the pathological damage of type 1 diabetes mellitus (IDDM)