泰国野葛 Pueraria mirifica 在泰国民间作为复壮药已有悠久历史。曾从该植物中分离到一独特的雌激素样酚类物质 miroe-strol(1)。作者以生物检测为导向,从泰国野葛中分得一个新的植物雌激素葛雌素(de-oxymiroestrol)(2),同时分得化合物1及其异构体 isomiroestrol(3)。泰国野葛根干燥粉碎,依次以己烷、乙酸乙酯和乙醇于索氏提取器中提取10 h。乙酸乙酯提取物经硅胶柱层析,以己烷-氯仿 Thailand Pueraria mirifica has a long history as a rejuvenator in Thailand. A unique estrogen-like phenolic substance, miroe-strol (1), has been isolated from this plant. The authors used bioassay as a guide to obtain a new phytoestrogenic de-oxymiroestrol (2) from Thai kudzu (Puerule spp.), and also identified compound 1 and its isomer isomiroestrol (3). Thai Pueraria lobata was ground and crushed and extracted with hexane, ethyl acetate and ethanol in Soxhlet extractor for 10 h. The ethyl acetate extract was subjected to silica gel column chromatography with hexane-chloroform