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目的探讨通过荧光原位杂交(fluorescencein situhybridization,FISH)检测3、7、9、17号染色体数目畸变诊断国人膀胱尿路上皮癌的可行性和有效性。方法采用3、7、17号染色体着丝粒探针和9p21区带探针对140例患者尿液脱落细胞核行荧光原位杂交,并同时行经腹部超声和尿脱落细胞学检查,以膀胱镜病理检查确诊,比较分析各种方法的敏感性和特异性。结果肿瘤组80例膀胱尿路上皮癌全部经病理检查确诊,其中68例FISH结果阳性,26例尿脱落细胞学阳性,71例经腹部超声诊断为膀胱癌。②非肿瘤对照组30例,其中29例FISH结果阴性,1例假阳性;尿脱落细胞学检查全部阴性;经腹超声检查23例阴性,7例假阳性。FISH、尿脱落细胞学、经腹部超声的灵敏度分别为85.00%、32.50%、88.75%,特异度分别为96.67%、100.00%、76.67%。结论使用FISH技术检测尿脱落细胞3、7、9、17号染色体数目畸变具有敏感度高、特异性强的优点,并具无创性,对国人膀胱尿路上皮癌的诊断具有重要的临床应用价值。
Objective To investigate the feasibility and effectiveness of fluorescence in situ hybridization (FISH) to detect the number of chromosome aberrations in chromosome 3, 7, 9 and 17 in the diagnosis of bladder cancer in Chinese people. Methods 140 cases of urine exfoliated cells were examined by fluorescence in situ hybridization using centromere probes 3, 7, 17 and 9p21 zone probe. At the same time, they were examined by cystoscopy and cystoscopy with cystoscopy Check the diagnosis, comparative analysis of the sensitivity and specificity of various methods. Results All the 80 cases of bladder urothelial carcinoma in the tumor group were diagnosed by pathology. Among them, 68 cases were positive for FISH, 26 cases were positive for exfoliative cytology, and 71 cases were diagnosed as bladder cancer by abdominal ultrasonography. ② Non-tumor control group of 30 cases, of which 29 cases of negative FISH results, 1 case of false positive; urine cytology all negative; transabdominal ultrasonography was negative in 23 cases, false positive in 7 cases. The sensitivity of FISH, exfoliative cytology and transabdominal ultrasound were 85.00%, 32.50% and 88.75% respectively, and the specificity were 96.67%, 100.00% and 76.67% respectively. Conclusion The detection of chromosome aberration in exfoliated cells 3, 7, 9 and 17 using FISH has the advantages of high sensitivity and specificity and is noninvasive, which has important clinical value in the diagnosis of bladder cancer .