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目的:研究补肾疏肝方对小鼠Lewis肺癌的抑制作用并探讨可能存在的免疫及内分泌机制。方法:选取荷瘤成功的小鼠40只,随机分为4组,分别为模型组、顺铂组、中药组、综合组。分别给予生理盐水(0.4 mL)、顺铂(0.5 mL,0.2 g.L-1)、补肾疏肝方中药(0.4 mL,1.75 g.L-1)、中药联合顺铂干预14 d。于第15天摘眼球取血、剥取肿瘤称质量、计算抑瘤率。采取酶联免疫吸附(ELISA)的方法检测血清白细胞介素-2(IL-2)、白细胞介素-10(IL-10)、雌二醇(E2)、睾酮(T)含量。结果:中药组、综合组、顺铂组抑瘤率及肺转移灶抑制率方面均显著高于模型组,对比具有统计学意义(P<0.05);中药组、顺铂组、综合组荷瘤小鼠血清IL-2,T水平升高;血清IL-10,E2水平降低,与模型组比较均有意义(P<0.05)。结论:补肾疏肝方可以抑制肿瘤生长,降低肺转移灶数目;具有调节免疫细胞因子IL-2,IL-10的作用,从而提高荷瘤小鼠的免疫力;可以调节荷瘤小鼠内分泌环境,增强小鼠抗肿瘤的作用。
Objective: To study the inhibitory effect of Bushen Shugan Recipe on mice Lewis lung carcinoma and to explore possible immune and endocrine mechanisms. Methods: Forty successful mice were randomly divided into 4 groups: model group, cisplatin group, traditional Chinese medicine group and comprehensive group. (0.4 mL), cisplatin (0.5 mL, 0.2 g.L-1) and Bushen Shugan Fang (0.4 mL, 1.75 g.L-1) respectively. On the 15th day, the eyeball was taken for blood, and the tumor was stripped to weigh the tumor, and the tumor inhibition rate was calculated. Serum interleukin-2, interleukin-10, estradiol and testosterone were detected by enzyme linked immunosorbent assay (ELISA). Results: The inhibitory rate of tumor and the inhibition rate of lung metastasis in Chinese medicine group, comprehensive group and cisplatin group were significantly higher than those in model group (P <0.05), while those in traditional Chinese medicine group, cisplatin group and combination group were significantly higher than those in model group The level of IL-2 and T in serum of mice increased, while the level of serum IL-10 and E2 decreased, which was significant compared with model group (P <0.05). Conclusion: Bushen Shugan decoction can inhibit tumor growth and reduce the number of lung metastases; regulate the immune cytokines IL-2, IL-10, thereby enhancing the immunity of tumor-bearing mice; can regulate tumor-bearing mice endocrine environment , Enhance the anti-tumor effect of mice.