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在常规肿瘤病理检验中,有时候由于组织固定不适当,会出现淋巴瘤切片染色不够清晰的问题,影响了恶性淋巴瘤的诊断工作。我们参考国外文献,采用B_5固定液重新固定组织切片的方法,改善了淋巴瘤组织切片的微细结构,使不易诊断的淋巴瘤得到了诊断。现将该法介绍如下。 1 材料和方法 1.1 试剂配制 B_5固定液:贮备液:氯化汞108;醋酸钠12.5g;蒸馏水1000ml。工作液:B_5贮备液90ml;甲醛液10ml。 1.2 方法 取活体标本检查中染色不满意的淋巴组织蜡块,切片4Pm,烘烤后脱蜡,各级梯度乙醇至
In the pathological examination of conventional tumors, sometimes due to improper tissue fixation, the problem of staining of lymphoma sections is not clear enough, which affects the diagnosis of malignant lymphomas. We refer to the foreign literature, using B_5 fixative to re-implant the tissue section to improve the fine structure of the lymphoma tissue section, making it difficult to diagnose lymphoma. The law is introduced as follows. 1 Materials and methods 1.1 Reagent preparation B_5 fixative: stock solution: Mercuric chloride 108; Sodium acetate 12.5g; Distilled water 1000ml. Working solution: B_5 stock solution 90ml; formaldehyde 10ml. 1.2 Methods Take samples of unsatisfied lymphoid tissue stained in biopsy specimens, slice 4Pm, dewax after baking, grade ethanol to gradient