论文部分内容阅读
目的:探讨清化瘀毒方对酒精性大鼠血清肝纤维化指标及组织金属蛋白酶抑制剂-1(TIMP-1)的作用机制。方法:采用以酒精为主导的复合因素建立酒精性大鼠肝纤维化模型,实验初随机分成正常对照组10只,造模组55只;造模成功后,造模组采用随机分成3组,分别为复方鳖甲软肝片组10只,清化瘀毒方组10只和模型空白组10只;于第20周末次给药后禁食,麻醉后打开腹腔,抽取腹主动脉血并离心检测AST、ALT、HA、LN、PCⅢ及TIMP-1的含量。结果:清化瘀毒方组AST、ALT、HA、LN、PCⅢ含量比模型空白组下降,有统计学意义(P<0.05);血清TIMP-1的表达清化瘀毒方组与模型空白组相比,有统计学意义(P<0.05)。结论:清化瘀毒方能改善肝功能,使血清中的肝纤维化指标下调,降低TIMP-1的蛋白表达升高,具有抗炎保肝、抗肝纤维化作用。
Objective: To investigate the mechanism of Qinghuayuxijian on serum hepatic fibrosis indexes and tissue inhibitor of metalloproteinase-1 (TIMP-1) in alcoholic rats. Methods: Alcohol-induced liver fibrosis model was established by alcohol-induced composite factors. The experiment was randomly divided into normal control group (n = 10) and modeling group (n = 55). After the model was successfully established, the model group was randomly divided into three groups They were Fufang Biejia Rugan Decoction 10, Qinghua Yugan Decoction 10 and model blank 10, respectively. After the last administration at the end of the 20th week, the rats were fasted, the abdominal cavity was opened after anesthesia, abdominal aorta blood was drawn and centrifuged The contents of AST, ALT, HA, LN, PCⅢ and TIMP-1 were detected. Results: The contents of AST, ALT, HA, LN and PCⅢ in QingHuaYuXu prescription group decreased significantly compared with the blank control group (P <0.05). The expression of TIMP-1 in QingHuaXueDu prescription group was significantly lower than that in blank control group Compared with the statistical significance (P <0.05). Conclusion: Qinghua Huayu decoction can improve liver function, reduce the serum hepatic fibrosis index, decrease the expression of TIMP-1 protein, and have anti-inflammation and liver-protection and anti-hepatic fibrosis.