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目的： 利用ｐｃＤＮＡ３ 质粒作为载体， 在人宫颈癌细胞（ＨｅＬａ 细胞） 中高效表达恶性疟原虫环子孢子蛋白 （ＣＳＰ）， 观察表达产物诱导ＢＡＬＢ／ｃ小鼠免疫的应答水平。方法： 目的基因ＰｆＣＳＰ在ＨｅＬａ 细胞中表达， 将纯化的表达产物免疫接种小鼠， 通过ＥＬＩＳＡ、Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ 分析、Ｔ 淋巴细胞增殖实验、ＮＫ 细胞活性检测和Ｔ淋巴细胞亚群测定， 观察其诱导ＢＡＬＢ／ｃ小鼠产生体液免疫和细胞免疫的应答水平。结果： ＥＬＩＳＡ 检测抗体滴度达１∶６ ４００；Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ 结果在３８．３ ｋＤａ 相应位置出现较清晰的显色条带；表达产物能特异刺激小鼠脾淋巴细胞增殖、ＣＤ４＋ 与ＣＤ８＋ 细胞有所增加， 并能提高小鼠ＮＫ细胞活性。结论： 真核表达系统ｐｃＤＮＡ３－Ｐｆ／ＨｅＬａ表达产物能特异刺激小鼠产生体液免疫和细胞免疫的应答， 并提高小鼠ＮＫ细胞活性的作用
OBJECTIVE: To efficiently express Plasmodium falciparum circumsporozoite protein (CSP) in human cervical cancer cells (HeLa cells) by using pcDNA3 plasmid as a vector and to observe the response level of BALB / c mice immunized with the expressed product. Methods: The target gene PfCSP was expressed in HeLa cells. Immunized mice were immunized with the expressed product. ELISA, Western blotting analysis, T lymphocyte proliferation assay, NK cell activity test and T lymphocyte subsets were determined. / C mice produce humoral and cellular immune response levels. Results: The titer of McAb reached 1: 400 by ELISA. The result of Western blotting showed a clearer band at the corresponding position of 38.3 kDa. The expressed product could specifically stimulate the proliferation of mouse splenic lymphocytes, and the number of CD4 + and CD8 + cells Increase, and can improve mouse NK cell activity. CONCLUSION: The eukaryotic expression system pcDNA3-Pf / HeLa can specifically stimulate humoral and cellular immune responses in mice and increase the activity of NK cells in mice