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以基因外重复的回文因子(repetitiveextragenicpalindromic,REP)和肠杆菌基因间重复一致序列(enterobacterialrepetitiveintergenicconsensus,ERIC)的碱基顺序设计出的引物为引物,用PCR(polymerasechainreaction)技术分别扩增了8株快生型大豆根瘤菌(Sinorhizobium)的总DNA,得出了具有菌株特异性的扩增产物电泳图谱,称REP-ERICPCR指纹图谱,将所得图谱进行性状编码后,用计算机数值分类系统进行平均连锁聚类分析,得出这8个菌株的聚类树状图。这一聚类结果与用其它方法聚类结果基本一致,说明REP-ERICPCR是一种鉴别快生型大豆根瘤菌菌株的经济、快速而又可靠的新方法。
The primers designed by repetitiveextragenic palindromic (REP) and enterobacterialrepetitiveintergenic consensus (ERIC) base sequences were used as primers. Eight PCR products were amplified by polymerase chain reaction (PCR) Obtained from the total DNA of Sinorhizobium, obtained the strain-specific amplification product electrophoresis pattern, called REP-ERICPCR fingerprinting, the characterization of the resulting map coding, the use of computer numerical classification system average linkage poly Based on the class analysis, we obtained the dendrogram of these 8 strains. This clustering result is basically consistent with the clustering results by other methods, indicating that REP-ERICPCR is a new economical, rapid and reliable method to identify fast-growing soybean rhizobium strains.