论文部分内容阅读
目的研究亚硒酸钠对氯化镉诱导的大鼠肝脏端粒酶逆转录酶(TERT)mRNA、c-Myc蛋白、p53蛋白表达的影响。方法健康雄性SD大鼠随机分为6组,对照组、硒组(5 μmol／kg亚硒酸钠)、 5 μmol／kg氯化镉组、10μmol／kg氯化镉组、硒(5μmol／kg亚硒酸钠)+5 μβmol/kg氯化镉组、硒(5 μmol／kg 亚硒酸钠)+10 μmol／kg氯化镉组。每组动物5只,染毒48 h后取出肝脏,用RT-PCR方法检测TERP 基因的表达,用免疫组织化学方法检测c-Myc蛋白和p53蛋白的表达。结果与对照组比较,5μmol／L kg氯化镉组和10μmol/kg氯化镉组TERP表达增多,10 μmol／L kg氯化镉组c-Myc蛋白表达增多,5 μmol／Lkg氯化镉组和10μmol／L kg氯化镉组p53蛋白表达增多。TERT的表达,硒+10／μmol／L kg氯化镉组与10 μmol／L kg氯化镉组比较降低;硒+10 μmol／Lkg氯化镉组比10μmol／Lkg氯化镉组c-Myc蛋白表达降低;硒+5 μmol／Lkg氯化镉组、硒+10 μmol／L kg氯化镉组与相对应的镉组比较p53蛋白表达降低。结论镉在5～10 μmol／L kg的剂量条件下可以诱导大鼠肝脏高表达TERT、c-Myc蛋白和p53蛋白,而硒对镉引起的TERT、c-Myc蛋白和p53蛋白的表达增高具有一定的拮抗作用。
Objective To study the effect of sodium selenite on cadmium chloride-induced hepatic telomerase reverse transcriptase (TERT) mRNA, c-Myc protein and p53 protein expression. Methods Healthy male SD rats were randomly divided into 6 groups: control group, selenium group (5 μmol / kg sodium selenite), 5 μmol / kg cadmium chloride group, 10 μmol / kg cadmium chloride group, Sodium selenite) +5 μβmol / kg cadmium chloride group, selenium (5 μmol / kg sodium selenite) +10 μmol / kg cadmium chloride group. Five animals in each group were sacrificed and the liver was removed 48 hours after exposure. The expression of TERP gene was detected by RT-PCR and the expressions of c-Myc and p53 were detected by immunohistochemistry. Results Compared with the control group, the expression of TERP was increased in 5μmol / L kg cadmium chloride group and 10μmol / kg cadmium chloride group, while the expression of c-Myc protein was increased in 10 μmol / L kg cadmium chloride group and 5 μmol / L kg cadmium chloride group And 10μmol / L kg cadmium chloride group p53 protein expression increased. TERT expression in selenium + 10 / μmol / L kg cadmium chloride group was lower than that in 10 μmol / L kg cadmium chloride group. Compared with 10 μmol / L kg cadmium chloride group, The expression of p53 protein was decreased in selenium + 5 μmol / L kg cadmium chloride group and selenium + 10 μmol / L kg cadmium chloride group compared with the corresponding cadmium group. Conclusions Cadmium induced a high expression of TERT, c-Myc protein and p53 protein in rat liver under the dose of 5 ~ 10 μmol / L kg, while selenium up-regulated the expression of TERT, c-Myc protein and p53 protein induced by cadmium Certain antagonism.