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本文旨在观察全身振动对去卵巢骨质疏松大鼠骨髓细胞Wnt/β-catenin信号通路的影响。将36只3月龄雌性Sprague Dawley(SD)大鼠,按体重随机分为假手术、去卵巢静止和去卵巢振动组。去卵巢10周后,去卵巢振动组大鼠每天接受2次振动(90Hz)刺激,每次15min。振动处理8周后,用双能X线骨密度仪在体检测大鼠体成分和骨密度,用Western blot检测骨髓细胞和骨髓基质细胞β-catenin和p-GSK3β蛋白的表达。结果显示,去卵巢振动组大鼠体脂重量和体脂含量显著低于去卵巢静止组,瘦体含量显著高于去卵巢静止组。去卵巢振动组大鼠胫骨近端骨密度显著高于去卵巢静止组,而全身、股骨远端和腰椎骨密度与去卵巢静止组相比无显著性差异。去卵巢振动组和去卵巢静止组的骨髓基质细胞β-catenin蛋白表达之间无显著性差异。去卵巢振动组骨髓细胞p-GSK3β蛋白的表达水平显著高于去卵巢静止组,而两组的骨髓基质细胞p-GSK3β蛋白表达之间无显著性差异。以上结果提示,全身振动能减缓去卵巢大鼠骨量的丢失,其机制涉及骨髓细胞p-GSK3β蛋白表达的上调。
The purpose of this paper is to investigate the effects of whole body vibration on Wnt / β-catenin signaling in bone marrow cells of ovariectomized rats. Thirty-six 3-month-old female Sprague Dawley rats were randomly divided into sham operation group, ovarieal group and ovariectomized group. After ovariectomized for 10 weeks, rats in ovariectomized group received 2 times of vibration (90 Hz) stimulation every day for 15 minutes. After vibration treatment for 8 weeks, the body composition and bone mineral density (BMD) of the rats were examined by dual-energy X-ray absorptiometry. The expressions of β-catenin and p-GSK3β in bone marrow cells and bone marrow stromal cells were detected by Western blot. The results showed that body fat mass and body fat content of ovariectomized rats were significantly lower than those of ovariectomized group, lean body mass was significantly higher than that of ovariectomized group. In the ovariectomized group, the proximal tibia bone mineral density was significantly higher in ovariectomized group than in ovariectomized group, while there was no significant difference in total body, distal femur and lumbar BMD compared with ovariectomized group. There was no significant difference in the expression of β-catenin protein in bone marrow stromal cells between ovariectomized group and ovariectomized group. The expression level of p-GSK3β protein in ovariectomized group was significantly higher than that in ovariectomized group, but the expression of p-GSK3β protein in bone marrow stromal cells was not significantly different between the two groups. The above results suggest that systemic vibration can slow the loss of bone mass in ovariectomized rats, and its mechanism involves the up-regulation of p-GSK3β protein expression in bone marrow cells.