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目的用pcDNA_3－P30真核表达质粒直接免疫小鼠，观察所诱导的小鼠细胞免疫应答。方法大量制备量级质粒pcDNA_3－P30，经肌肉注射BALB／c小鼠，每隔3周接种1次，一共免疫3次。用MTT方法对脾脏的NK杀伤细胞率和淋巴细胞的转化率进行测定，采用免疫荧光法对CD4+、CD8+细胞进行测定。结果实验组NK细胞杀伤率为：70．0％±3．64，对照组及空白对照组分别为48．5％±6．06和470％±5．93，实验组NK细胞活性比对照组明显增高（P＜0．05）；ConA刺激小鼠淋巴细胞转化实验，实验组与对照组及空白对用级差异无显著性（P＞0．05）;对T淋巴细胞亚群CD4＋、CD8＋进行动态分析，可见随着感染时间的延长，CD8＋的数量逐渐上升，CD4＋／CD8＋的比率逐渐下降，实验组与对照组及空白对照有明显差异（P＜0．05）。结论重组质粒pcDNA3-p30免疫BALB／c小鼠可诱导一定的细胞免疫。
Objective To directly immunize mice with pcDNA_3-P30 eukaryotic expression plasmid to observe the induced cellular immune response in mice. Methods The plasmid pcDNA_3-P30 was prepared in large quantities. BALB / c mice were injected intramuscularly and immunized once every 3 weeks for 3 times. The NK cell killing rate and lymphocyte transformation rate of the spleen were measured by MTT method. The CD4 + and CD8 + cells were measured by immunofluorescence. Results The killing rate of NK cells in the experimental group was 70.0% ± 3.64, the control group and the blank control group were 48.5% ± 6.06 and 470% ± 5.93 respectively. The NK cell activity in the experimental group was significantly higher than that in the control group (P <0.05). ConA stimulated lymphocyte transformation in mice, there was no significant difference between the experimental group and the control group and the blank control group (P> 0.05). The T lymphocyte subsets CD4 +, CD8 + Dynamic analysis showed that with the extension of infection time, the number of CD8 + gradually increased and the ratio of CD4 + / CD8 + gradually decreased. There was significant difference between the experimental group and the control group and the blank control group (P <0.05). Conclusion Immunization of BALB / c mice with recombinant plasmid pcDNA3-p30 can induce certain cellular immunity.