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Ｙ Ｎ８７４４８ 毒株１９８６ 年分离自云南傣族５２ 岁女发热病人的血液标本，曾用血清学方法鉴定为披膜病毒科甲病毒属。用我们创立的单引物差异 Ｒ Ｔ－ Ｐ Ｃ Ｒ 方法及简并引物 Ｒ Ｔ－ Ｐ Ｃ Ｒ 法均已证明：该病毒为甲病毒属的辛德毕斯样病毒（ Ｓｉｎｄｂｉｓ － ｌｉｋｅ ｖｉｒｕｓ） 。本研究从甲病毒属的病毒基因序列的共同保守区，设计４ 对引物。用 Ｒ Ｔ－ Ｐ Ｃ Ｒ 方法扩增，再将扩增片段分别连结克隆到ｐ Ｇ Ｅ Ｍ－ Ｔ 载体。通过对４ 个克隆的测序完成了对 Ｙ Ｎ８７４４８ 毒株的全部结构区基因序列的测定，结果表明：（１） Ｙ Ｎ８７４４８ 病毒的全部结构区基因序列长度为４ ０５９ 个核苷酸（ 不包括多聚腺苷酸尾） ；（２） Ｙ Ｎ８７４４８ 病毒的全部结构区基因与辛德毕斯病毒家族中毒力最强的、唯一能致成年小鼠１００ ％ 死亡的、南非分离的辛德毕斯样病毒 Ｓ Ａ Ａ Ｒ８６ 株相应基因的核苷酸序列同源性为９９ ％ ，但 Ｙ Ｎ８７４４８ 毒株不致成年小鼠死亡；（３） 二者基因结构上有一点大的差异是， Ｙ Ｎ８７４４８ 病毒位于基因组８ ６４１ｂｐ 处的结构区基因 Ｅ２ 和 Ｅ３ 之间多出３ 个核苷酸（ Ａ Ａ Ａ） ；（４） 与其它甲属病毒的进化树比较来看?
Y N87448 strain A blood specimen from a 52-year-old female with fever in Yunnan’s Dai nationality in 1986 was identified as a togavirus by the serological method. It has been demonstrated that the virus is a Sindbis-like virus of the alphavirus genus using the single-primer differential RT-PCR method and the degenerate primer RT-PCR method that we have established. In this study, four pairs of primers were designed from the common conserved region of the alphavirus gene sequences. Amplified with RTPCR method, and the amplified fragments were respectively linked and cloned into pGEM-T vector. The complete structural region of Y N87448 strain was sequenced by sequencing the four clones. The results showed that: (1) The entire structural region of Y N87448 virus has a length of 4 059 nucleotides (excluding multiple Poly (A) tail); (2) the entire structural region of the Y N87448 virus is the most virulent to the Sindbis virus family and is the only Sindbis-like virus S A The nucleotide sequence homology of the corresponding gene of A R86 strain was 99%, but the strain Y N87448 did not cause death in adult mice. (3) One of the structural differences between the two genes was that Y N87448 virus was located in genome 8 641bp at the structure of the gene E2 and E3 more than three nucleotides (A A A); (4) compared with other Meta viruses phylogenetic tree comparison?